Microfluidic devices for the generation of centimetre-long motor nerve organoids derived from iPSCs

Micro & nano letters

article

Shohei Kaneda¹  Kohei Kurihara¹  Kenji Suzuki¹  Hideaki Takanobu¹  Seii Ohka² 

[1] Department of Mechanical Systems Engineering, Faculty of Engineering, Kogakuin University;Tokyo Metropolitan Institute of Medical Science

关键词: microfluidics;    cellular biophysics;    bioMEMS;    neurophysiology;    microfabrication;    biomedical equipment;    fascicle length;    microfluidic devices;    centimetre-long motor nerve organoids;    microfluidic neuronal culture technique;    microchannel structure;    motor neuronal axons;    human induced pluripotent stem cells;    microchannel length;    centimetre-long axonal fascicles;    microchannel height;    motor neuronal cells;    human iPSC cell line;    neurospheres;    polydimethylsiloxane chip;    glass substrate;    N2B27 medium;    size 120.0 mum;    size 150.0 mum;    size 220.0 mum;    size 2.0 cm;    size 1.25 cm;    size 20 mm;   

DOI  :  10.1049/mnl.2019.0541

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Although a microfluidic neuronal culture technique that utilised a microchannel structure to generate a motor nerve organoid consisted of neurospheres and fascicles of motor neuronal axons derived from human induced pluripotent stem cells (iPSCs) was reported recently, the lengths of the axonal fascicles were limited to less than 1 cm, which corresponded to the microchannel length. In this study, the authors fabricated microfluidic devices composed of a polydimethylsiloxane chip with a glass substrate and 20-mm-long and 150-μm-wide microchannels to obtain centimetre-long axonal fascicles. They investigated the effects of microchannel height on fascicle length by using three different microchannel heights (120, 150, and 220 μm), neurospheres containing 80,000 motor neuronal cells derived from a human iPSCs cell line (409B2), and N2B27 medium. Maximum and average fascicle lengths of 2 and 1.25 cm, respectively, were successfully achieved with 150-μm-high microchannels. The fascicles grew in length, with an increase in the number of days spent in the culture (ranging from 0.44 to 0.60 mm per day), growing to over 1 cm around the 23rd day of culture. The difference in fascicle length obtained with 120-μm and 150-μm-high microchannels suggests that height significantly affects the length of the axonal fascicle of the nerve organoid.

【 授权许可】

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