Biological particle manipulation: an example of Jurkat enrichment

Micro & nano letters

article

Emre Altinagac¹  Huseyin Kizil¹  Levent Trabzon¹ 

[1] Department of Nanoscience & Nanoengineering, Istanbul Technical University;Department of Metallurgical and Materials Engineering, Istanbul Technical University;Department of Mechanical Engineering, Istanbul Technical University

关键词: lab-on-a-chip;    cellular biophysics;    polymers;    electrodes;    electrophoresis;    microfluidics;    bioMEMS;    biological particle manipulation;    Jurkat cell enrichment;    dielectrophoresis-based separation;    microparticle flow behaviour;    living cell;    microparticle flow simulation;    COMSOL Multiphysics 4.3a software;    lab-on-a-chip device;    titanium-interdigitated electrode layer;    glass substrate;    polydimethylsiloxane;    PDMS microchannel fabrication;    microparticle separation;    green polystyrene particle;    red polystyrene particle;    size 3.2 mum;    size 9.8 mum;   

DOI  :  10.1049/mnl.2015.0185

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

HoD, Pediatric Surgery, KRL Hospital, G-9/1, Islamabad; E-mail: [e-mail] Congenital Torticollis (CT) often occurs in those children who develop sternomastoid tumor (a benign condition) in neonatal period, which is a hard swelling in the substance of one of the sternomastoid muscle. It gradually involutes and disappears spontaneously without requiring any treatment. But it leaves varying degrees of fibrosis resulting in contracture of the muscle, leading to tilting of the neck on the ipsilateral side. If the tumor is diagnosed early and physiotherapy by stretching exercises is commenced early, the development of CT can be avoided in majority of children. But often tumor is missed, physiotherapy is frequently inadequate and parents may not even notice a tilted neck. The result is that the patient presents late with several changes in the face, and neck, termed ‘facial hpoplasia’, and ‘plagicephaly’ in the shape of ipsilateral flat skull. In late and neglected cases eyesight may also be affected.If conservative treatment has failed, surgery should be performed as soon as possible after the age of one year to avoid permanent sequel. After surgery a harness or a cervical collar should be worn for some months and physiotherapy continued. The patient may be difficult to be mask ventilated and to be positioned optimally for intubation.Surgery entails division of contractured sternomastoid muscle, prevertebral shrunken fascia and connective tissue. This can be done in three places, e.g. behind the ear, middle part of the sternomastoid or more commonly just above the sternum and clavicle.

【 授权许可】

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