Development and optimisation of hepatitis B recombinant antigen loaded chitosan nanoparticles as an adjuvant using the response surface methodology

Micro & nano letters

article

Mohsen Mehrabi¹  Shima Sadeghi-Soureh²  Naser Mohammadpour Dounighi³  Seyed Mahdi Rezayat⁴  Delaram Doroud⁶  Mehdi Khoobi⁷  Amir Amani⁴ 

[1] Department of Medical Nanotechnology, School of Medicine, Shahroud University of Medical Sciences;Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences;Razi Vaccine and Serum Research Institute, Agricultural Research;Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences;Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences;Regulatory Department, Production and Research Complex, Pasteur Institute of Iran;Biomaterials Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences

关键词: electrokinetic effects;    drug delivery systems;    nanomedicine;    proteins;    drugs;    pH;    nanofabrication;    biochemistry;    encapsulation;    particle size;    polymers;    biomedical materials;    response surface methodology;    cellular biophysics;    nanoparticles;    molecular biophysics;    optimisation;    optimisation;    hepatitis B recombinant antigen loaded chitosan nanoparticles;    response surface methodology;    rHBsAg-CS NPs;    hepatitis B vaccine;    RSM;    ionic gelation technique;    tripolyphosphate;    antigen concentration;    loading capacity;    optimised preparation;    optimised NPs;    positive zeta potential;    in vitro release profile;    optimised formulation;    slow sustained antigen release;    concentration-dependence;    released antigen;    antigenicity;    pH;    homogenisation speed;    encapsulation efficiency;    spherical shape;    particle size;    cell viability assay;    toxicity;    SDS-PAGE analysis;    structural stability;    ELISA;    Ouchterlony double immunodiffusion tests;   

DOI  :  10.1049/mnl.2019.0355

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

The main purpose of this Letter was to develop and optimise recombinant hepatitis B antigen-loaded chitosan nanoparticles (rHBsAg-CS NPs) as a new adjuvant for hepatitis B vaccine through designing by response surface methodology (RSM). The NPs were prepared by employing ionic gelation technique. RSM was utilised by selecting the independent variablesincluding the concentration of CS, tripolyphosphate and antigen concentration as well as pH and homogenisation speed to obtain maximum encapsulation efficiency (EE) and loading capacity (LC). EE and LC of the optimised preparation were 93.2 ± 1.1 and 25.6 ± 1.38%, respectively. Optimised NPs showed spherical shape, particle size of 187 ± 14 nm and positive zeta potential (+31.3 ± 1.5 mV). In vitro release profile of optimised formulation revealed an initial burst, followed by a slow sustained antigen release. Cell viability assay indicated that the toxicity of NPs was concentration-dependent. SDS-PAGE analysis confirmed the structural stability and integrity of the released antigen. ELISA and Ouchterlony double immunodiffusion tests of released antigen showed that the antigenicity was preserved during the process of NP formation.

【 授权许可】

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