Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms

Microbial Cell Factories

Wenqian Xu¹  Zhenyu Zhang¹  Pengfu Liu¹  Xiaohe Chu¹  Weike Su²  Huawei Zhang³ 

[1] Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People’s Republic of China;Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People’s Republic of China;School of Pharmaceutical Sciences, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People’s Republic of China;School of Pharmaceutical Sciences, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People’s Republic of China;

关键词: Trans;    Biosynthetic pathway;    Proline hydroxylases;    Metabolic engineering;   

DOI  :  10.1186/s12934-021-01579-2

来源: Springer

【 摘 要 】

Trans-4-hydroxy-l-proline is an important amino acid that is widely used in medicinal and industrial applications, particularly as a valuable chiral building block for the organic synthesis of pharmaceuticals. Traditionally, trans-4-hydroxy-l-proline is produced by the acidic hydrolysis of collagen, but this process has serious drawbacks, such as low productivity, a complex process and heavy environmental pollution. Presently, trans-4-hydroxy-l-proline is mainly produced via fermentative production by microorganisms. Some recently published advances in metabolic engineering have been used to effectively construct microbial cell factories that have improved the trans-4-hydroxy-l-proline biosynthetic pathway. To probe the potential of microorganisms for trans-4-hydroxy-l-proline production, new strategies and tools must be proposed. In this review, we provide a comprehensive understanding of trans-4-hydroxy-l-proline, including its biosynthetic pathway, proline hydroxylases and production by metabolic engineering, with a focus on improving its production.

【 授权许可】

CC BY