Streptozotocin induces alpha-2u globulin nephropathy in male rats during diabetic kidney disease

BMC Veterinary Research

Kanchana Kengkoom¹  Wannee Angkhasirisap¹  Naphatson Phansom²  Rongdej Tungtrakanpoung²  Khwanchanok Tuentam²  Tapanee Kanjanapruthipong³  Sumate Ampawong³ 

[1] Academic Service Division, National Laboratory Animal Center, Mahidol University, 999, Salaya, Puttamonthon, 73170, Nakorn Pathom, Thailand;Department of Biology, Faculty of Science, Naresuan University, 99, Moo 9, Phitsanulok-NakornSawan Road, 65000, Phitsanulok, Thailand;Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, 420/6, Ratchawithi Road, Ratchathewi, 10400, Bangkok, Thailand;

关键词: Alpha-2u globulin;    Diabetic kidney disease;    Histopathology;    Nephropathy;    Streptozotocin;   

DOI  :  10.1186/s12917-021-02814-z

来源: Springer

【 摘 要 】

BackgroundAlpha-2u globulin nephropathy mainly shows toxicological pathology only in male rats induced by certain chemicals and drugs, such as levamisole (antiparasitic and anticancer drugs). Streptozotocin (STZ) is also an anticancer-antibiotic agent that has been used for decades to induce a diabetic kidney disease model in rodents. The purpose of this study is to determine if STZ causes alpha-2u globulin nephropathy in male rats during an advanced stage of diabetic kidney disease. Alpha-2u globulin nephropathy, water absorption and filtration capacities (via aquaporin [AQP]-1, − 2, − 4 and − 5) and mitochondrial function (through haloacid dehalogenase-like hydrolase domain-containing protein [HDHD]-3 and NADH-ubiquinone oxidoreductase 75 kDa subunit [NDUFS]-1 proteins) were examined in STZ-induced diabetic Wistar rat model.ResultsMore than 80% of severe clinical illness rats induced by STZ injection simultaneously exhibited alpha-2u globulin nephropathy with mitochondrial degeneration and filtration apparatus especially pedicels impairment. They also showed significantly upregulated AQP-1, − 2, − 4 and − 5, HDHD-3 and NDUFS-1 compared with those of the rats without alpha-2u globulin nephropathy.ConclusionsSTZ-induced alpha-2u globulin nephropathy during diabetic kidney disease in association with deterioration of pedicels, renal tubular damage with adaptation and mitochondrial driven apoptosis.

【 授权许可】

CC BY