Effects of chronic Porphyromonas gingivalis lipopolysaccharide infusion on skeletal muscles in mice

The journal of physiological sciences

article

Naoya Kawamura¹  Yoshiki Ohnuki¹  Ichiro Matsuo¹  Kenji Suita¹  Misao Ishikawa³  Yasumasa Mototani¹  Kouichi Shiozawa¹  Aiko Ito¹  Yuka Yagisawa¹  Yoshio Hayakawa¹  Megumi Nariyama⁶  Daisuke Umeki⁴  Yuko Ujiie²  Kazuhiro Gomi²  Satoshi Okumura¹ 

[1] Department of Physiology, Tsurumi University School of Dental Medicine;Department of Periodontology, Tsurumi University School of Dental Medicine;Department of Oral Anatomy, Tsurumi University School of Dental Medicine;Department of Orthodontics, Tsurumi University School of Dental Medicine;Department of Dental Anesthesiology, Tsurumi University School of Dental Medicine;Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine

关键词: Lipopolysaccharide;    Periodontitis;    Sarcopenia;    Apoptosis;    Fibrosis;    Signal transduction;   

DOI  :  10.1007/s12576-019-00670-z

来源: Springer

【 摘 要 】

Periodontitis, which is caused by various oral organisms, predominantly affects adults, and is one of the main causes of tooth loss, as well as leading to progression of numerous systemic diseases. However, its relationship to sarcopenia (aging-associated degenerative loss of skeletal muscle mass and function) remains unclear. The aim of this study was to investigate the effects of Porphyromonas gingivalis lipopolysaccharide (PG-LPS) on skeletal muscle in mice, and to establish the underlying mechanisms. Mice (C57BL/6) were injected with PG-LPS (0.8 mg/kg/day) for 4 weeks. This treatment significantly decreased the weight of fast-twitch skeletal muscles (masseter and tibialis anterior muscles), but not that of slow-twitch skeletal muscle (soleus muscle). The area of fibrosis was significantly increased in masseter muscle, but remained unchanged in the other two muscles. The number of apoptotic myocytes was significantly increased (approximately eightfold) in masseter muscle. These data suggest that persistent subclinical exposure to PG-LPS might reduce the size of fast-twitch skeletal muscle, but not slow-twitch skeletal muscle. Masseter muscle appears to be especially susceptible to the adverse effects of PG-LPS, because muscle remodeling (muscle fibrosis and myocyte apoptosis) was induced solely in masseter muscle. Thus, periodontitis might be one of the major causes of oral sarcopenia.

【 授权许可】

Unknown