The ectonucleoside triphosphate diphosphohydrolase-2 (NTPDase2) in human endometrium: a novel marker of basal stroma and mesenchymal stem cells

Purinergic signalling

article

Carla Trapero¹  August Vidal¹  Aitor Rodríguez-Martínez¹  Jean Sévigny⁴  Jordi Ponce²  Buenaventura Coroleu⁵  Xavier Matias-Guiu²  Mireia Martín-Satué¹ 

[1] Campus Bellvitge;Institut d’Investigació Biomèdica de Bellvitge (IDIBELL), Oncobell Program;Hospital Universitari de Bellvitge;Centre de Recherche du CHU de Québec, Université Laval;Hospital Universitari Quiron Dexeus

关键词: Purinergic signaling;    Adenomyosis;    SUSD2;    MSC;    Ectonucleotidases;   

DOI  :  10.1007/s11302-019-09656-3

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

The human endometrium undergoes repetitive regeneration cycles in order to recover the functional layer, shed during menses. The basal layer, which remains in charge of endometrial regeneration in every cycle, contains adult stem or progenitor cells of epithelial and mesenchymal lineage. Some pathologies such as adenomyosis, in which endometrial tissue develops within the myometrium, originate from this layer. It is well known that the balance between adenosine triphosphate (ATP) and adenosine plays a crucial role in stem/progenitor cell physiology, influencing proliferation, differentiation, and migration. The extracellular levels of nucleotides and nucleosides are regulated by the ectonucleotidases, such as the nucleoside triphosphate diphosphohydrolase 2 (NTPDase2). NTPDase2 is a membrane-expressed enzyme found in cells of mesenchymal origin such as perivascular cells of different tissues and the stem cells of adult neurogenic regions. The aim of this study was to characterize the expression of NTPDase2 in human nonpathological cyclic and postmenopausic endometria and in adenomyosis. We examined proliferative, secretory, and atrophic endometria from women without endometrial pathology and also adenomyotic lesions. Importantly, we identified NTPDase2 as the first marker of basal endometrium since other stromal cell markers such as CD10 label the entire stroma. As expected, NTPDase2 was also found in adenomyotic stroma, thus becoming a convenient tracer of these lesions. We did not record any changes in the expression levels or the localization of NTPDase2 along the cycle, thus suggesting that the enzyme is not influenced by the female sex hormones like other previously studied ectoenzymes. Remarkably, NTPDase2 was expressed by the Sushi Domain containing 2 (SUSD2)+ endometrial mesenchymal stem cells (eMSCs) found perivascularly, rendering it useful as a cell marker to improve the isolation of eMSCs needed for regenerative medicine therapies.

【 授权许可】

CC BY