The fate of human SUSD2+ endometrial mesenchymal stem cells during decidualization

Stem Cell Research

Daniela Gold¹  Philipp Reif¹  Wolfgang Schoell¹  Tina Gorsek Sparovec¹  Gerit Moser²  Julia Feichtinger²  Julia Kargl³  Zala Nikita Mihalic³  Udo R. Markert⁴  Caroline E. Gargett⁵ 

[1] Department of Obstetrics and Gynaecology, Medical University of Graz, Austria;Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, Austria;Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz, Austria;Placenta Lab, Department of Obstetrics, Jena University Hospital, Jena, Germany;Ritchie Centre, Hudson Institute of Medical Research and Department of Obstetrics and Gynaecology, Monash University, Australia;

关键词: Endometrium;    Perivascular;    eMSC;    SUSD2;    Decidualization;   

DOI  :  

来源: DOAJ

【 摘 要 】

Regeneration of the endometrial stromal compartment in premenopausal women is likely maintained by the perivascular endometrial mesenchymal stem/stromal cells (eMSC) expressing sushi domain containing 2 (SUSD2). The fate of SUSD2+ eMSC during pregnancy and their role in decidualization is not fully known. The aim of our study was to determine the effect of progesterone on the stemness of the SUSD2+ eMSC isolated from non-pregnant uterine samples. Secondary objectives were to characterize the functional capacity including differentiation and clonogenicity assays of SUSD2+ eMSC isolated from decidua at full term and compare it to the capacity of those isolated from non-pregnant uterine samples. Progesterone treatment induced changes in the decidual gene expression profile in non-pregnant SUSD2+ eMSC. Data analysis of a publicly available single cell RNA-seq data set revealed differential expression of several mesenchymal and epithelial signature genes between the SUSD2+ eMSC and the decidual stromal cells, suggesting mesenchymal-to-epithelial transition occurs during decidualization. Histological analysis revealed a significantly lower abundance of SUSD2+ eMSC in 1st trimester and full term samples compared to non-pregnant samples, p = 0.0296 and 0.005, respectively. The differentiation and the colony forming capacity did not differ significantly between the cells isolated from non-pregnant and pregnant uterine samples. Our results suggest that SUSD2+ eMSC undergo decidualization in vitro, while maintaining MSC plasma membrane phenotype. Human eMSC seem to play an important role in the course of endometrial decidualization and embryo implantation. Pregnancy reduced the abundance of SUSD2+ eMSC, however eMSC function remains intact.

【 授权许可】

Unknown