The fate of human SUSD2+ endometrial mesenchymal stem cells during decidualization

Stem cell research

article

Tina Gorsek Sparovec¹  Udo R. Markert²  Philipp Reif¹  Wolfgang Schoell¹  Gerit Moser³  Julia Feichtinger³  Zala Nikita Mihalic⁴  Julia Kargl⁴  Caroline E. Gargett⁵  Daniela Gold¹ 

[1] Department of Obstetrics and Gynaecology, Medical University of Graz;Placenta Lab, Department of Obstetrics, Jena University Hospital;Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz;Otto Loewi Research Center, Division of Pharmacology, Medical University of Graz;Ritchie Centre, Hudson Institute of Medical Research and Department of Obstetrics and Gynaecology, Monash University

关键词: Endometrium;    Perivascular;    eMSC;    SUSD2;    Decidualization;   

DOI  :  10.1016/j.scr.2022.102671

学科分类：生理学

来源: Academic Press

【 摘 要 】

Regeneration of the endometrial stromal compartment in premenopausal women is likely maintained by the perivascular endometrial mesenchymal stem/stromal cells (eMSC) expressing sushi domain containing 2 (SUSD2). The fate of SUSD2+ eMSC during pregnancy and their role in decidualization is not fully known. The aim of our study was to determine the effect of progesterone on the stemness of the SUSD2+ eMSC isolated from non-pregnant uterine samples. Secondary objectives were to characterize the functional capacity including differentiation and clonogenicity assays of SUSD2+ eMSC isolated from decidua at full term and compare it to the capacity of those isolated from non-pregnant uterine samples. Progesterone treatment induced changes in the decidual gene expression profile in non-pregnant SUSD2+ eMSC. Data analysis of a publicly available single cell RNA-seq data set revealed differential expression of several mesenchymal and epithelial signature genes between the SUSD2+ eMSC and the decidual stromal cells, suggesting mesenchymal-to-epithelial transition occurs during decidualization. Histological analysis revealed a significantly lower abundance of SUSD2+ eMSC in 1 s t trimester and full term samples compared to non-pregnant samples, p  = 0.0296 and 0.005, respectively. The differentiation and the colony forming capacity did not differ significantly between the cells isolated from non-pregnant and pregnant uterine samples. Our results suggest that SUSD2+ eMSC undergo decidualization in vitro , while maintaining MSC plasma membrane phenotype. Human eMSC seem to play an important role in the course of endometrial decidualization and embryo implantation. Pregnancy reduced the abundance of SUSD2+ eMSC, however eMSC function remains intact.

【 授权许可】

CC BY|CC BY-NC-ND