eLife | |
Calcium signaling through a transient receptor channel is important for Toxoplasma gondii growth | |
Miryam Andrea Hortua Triana1  Nathan M Chasen1  Karla Marie Márquez-Nogueras2  Silvia NJ Moreno3  Ivana Y Kuo4  | |
[1] Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, United States;Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, United States;Department of Cell and Molecular Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, United States;Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, United States;Department of Cellular Biology, University of Georgia, Athens, United States;Department of Cell and Molecular Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, United States; | |
关键词: Toxoplasma gondii; calcium signaling; transient receptor channel; calcium entry; ER calcium; Other; | |
DOI : 10.7554/eLife.63417 | |
来源: eLife Sciences Publications, Ltd | |
【 摘 要 】
Transient receptor potential (TRP) channels participate in calcium ion (Ca2+) influx and intracellular Ca2+ release. TRP channels have not been studied in Toxoplasma gondii or any other apicomplexan parasite. In this work, we characterize TgGT1_310560, a protein predicted to possess a TRP domain (TgTRPPL-2), and determined its role in Ca2+ signaling in T. gondii, the causative agent of toxoplasmosis. TgTRPPL-2 localizes to the plasma membrane and the endoplasmic reticulum (ER) of T. gondii. The ΔTgTRPPL-2 mutant was defective in growth and cytosolic Ca2+ influx from both extracellular and intracellular sources. Heterologous expression of TgTRPPL-2 in HEK-3KO cells allowed its functional characterization. Patching of ER-nuclear membranes demonstrates that TgTRPPL-2 is a non-selective cation channel that conducts Ca2+. Pharmacological blockers of TgTRPPL-2 inhibit Ca2+ influx and parasite growth. This is the first report of an apicomplexan ion channel that conducts Ca2+ and may initiate a Ca2+ signaling cascade that leads to the stimulation of motility, invasion, and egress. TgTRPPL-2 is a potential target for combating toxoplasmosis.
【 授权许可】
CC BY
【 预 览 】
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