Phospholipase C and phosphatidylinositol 3-kinase signaling are involved in the exogenous arachidonic acid-stimulated respiratory burst in human neutrophils

Journal of Leukocyte Biology

Zhaoxia Liu¹  Xun Shen¹  Jiang Liu¹  Shaokun Chuai¹ 

[1] Institute of Biophysics, Chinese Academy of Sciences, BeijingInstitute of Biophysics, Chinese Academy of Sciences, BeijingInstitute of Biophysics, Chinese Academy of Sciences, Beijing

关键词: signal transduction;    calcium mobilization;    calcium entry;    U73122;    wortmannin;   

DOI  :  10.1189/jlb.1102537

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

To define the role of phospholipase C (PLC) and phosphatidylinositol 3-kinase (PI-3K), signaling pathways in arachidonic acid (AA)-stimulated respiratory burst in human neutrophils, the AA-stimulated respiratory burst, Ins(1,4,5)P3 production, PI-3K activation, and cytoplasmic Ca2+ mobilization were investigated. It was found that Ins(1,4,5)P3 production and PI-3K activity in AA-stimulated cells were increased in a dose-dependent manner. U73122, the PLC inhibitor, effectively inhibited the AA-stimulated respiratory burst and Ca2+ release from the intracellular calcium store but not the activity of PI-3K, indicating the independence of PI-3K signaling on PLC activation. Wortmannin, the PI-3K inhibitor, at the concentration sufficient to inhibit PI-3K activity, can only partially inhibit Ca2+ release from the internal store, indicating a partial regulation of PLC signaling by PI-3K and the existence of two pathways initiated by different PLC subfamilies. One is regulated by PI-3K activation, and the other is independent of PI-3K signaling. It was observed that AA could still induce a noncapacitative Ca2+ entry in the cells when Ca2+ release from the intracellular store was blocked by a PLC inhibitor, or a capacitative Ca2+ entry was induced by preincubation with thapsigargin. However, the AA-mediated, noncapacitative Ca2+ entry seems to play a little, if any, role in the stimulated respiratory burst. The present study suggests that the PLC signaling pathway, which may be activated by PLCβ and PLCγ, respectively, and the PI-3K signaling pathway are involved in the AA-stimulated respiratory burst in human neutrophil.

【 授权许可】

Unknown