Persistent increase in the amount of aquaporin‐5 in the apical plasma membrane of rat parotid acinar cells induced by a muscarinic agonist SNI‐2011

FEBS Letters

Skowronski, Mariusz T¹  Ishikawa, Yasuko¹  Ishida, Hajime¹ 

[1]Department of Pharmacology, School of Dentistry, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan

关键词: Apical plasma membrane;    Aquaporin-5;    Calcium oscillation;    M3 muscarinic receptor;    Parotid acinar cell;    SNI-2011;    SNI-2011;    (±)-cis-2-methylspilo(1;    3-oxathiolane-5;    3′) quinuclidine;    p-F-HHSiD;    hexahydro-sila-difenidol;    TMB-8;    4;    5-trimethoxy-benzoic 8-(diethylamino) octylester;    U73122;    1-6-[6-[[(17β)-3-methoxyestra-1;    3;    5-(10)-trien-17-yl]-amino]hexyl-1H-10ymole-2;    5-dione];    GF 109203X;    bisindolmaleimide;    PMA;    phorbol 12-myristate 13-acetate;    PKA;    protein kinase A;    PKC;    protein kinase C;    IP3;    inositol 1;    4;    5-trisphosphate;   

DOI  :  10.1016/S0014-5793(00)01763-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

SNI-2011 induces the long-lasting increase in the amount of aquaporin-5 (AQP5) in apical plasma membranes (APMs) of rat parotid acini in a concentration-dependent manner. This induction was inhibited by p-F-HHSiD, U73122, TMB-8, or dantrolene but not by bisindolmaleimide or H-7, indicating that SNI-2011 acting at M₃ muscarinic receptors induced translocation of AQP5 via [Ca²⁺]_i elevation but not via the activation of protein kinase C. In contrast, acetylcholine induced a transient translocation of AQP5 to APMs. SNI-2011 induces long-lasting oscillations of [Ca²⁺]_i in the presence of extracellular Ca²⁺. Thus, SNI-2011 induces a long-lasting translocation of AQP5 to APMs coupled with persistent [Ca²⁺]_i oscillations.

【 授权许可】

Unknown