Kaempferia galanga L. Rhizome As a Potential Dental Plaque Preventive Agent

ISCC (Indonesian Journal of Cancer Chemoprevention)

ARTICLE

Triana Hertiani¹  Sylvia Utami Tunjung Pratiwi¹  Iramie Duma Kencana Irianto¹  Aini Febriana¹ 

[1] Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 INDONESIA Faculty of Pharmacy

关键词: biofilm inhibitor;    antibacterial;    Kaempferia galanga;   

DOI  :  10.14499/indonesianjcanchemoprev1iss1pp19-25

学科分类：药学

来源: Indonesian Society for Cancer Chemoprevention

【 摘 要 】

Dental plaque prevention can be achieved by inhibition of mouth cavity microbes to built biofilm. Kaempferia galanga rhizome has been known as a potential antibacterial agent. This research aimed to reveal the potency of Kaempferia galanga extract and essential oil as anti plaque active agents, based on their in vitro inhibitory activity against the planktonic growth and biofilm of Streptococcus mutans ATCC 21752. Kaempferia galanga extract was obtained by defatting dried-pulverized samples in petroleum ether prior to immersion in 70% ethanol. The fresh rhizome was steam-hydro distilled for 6 h to yield the essential oil. Antibacterial and anti biofilm assays were measured by micro dilution technique on polystyrene 96-wells micro titer plates at 37°C. The percentage of inhibition was calculated by comparing the absorbance of samples to the vehicle (control) measured by micro plate reader at 595 nm. Biofilms formed were first stained by 1% crystal violet. The above assays were performed in triplicates. This study revealed that both K. galanga rhizome essential oil and ethanolic extract showed antibacterial and antibiofilm activity towards S. mutans . The ethanol extract showed MIC 90 value at 0.091% w / v and MBC at 2.724% w / v for antibacterial activity; IC 50 at 0.048 % w / v for anti biofilm formation activity; and EC 50 at 0.052% w / v for biofilm degradation activity. Until the highest concentration tested (0.6% w / v ), the MIC 90 and MBC values of the essential oil were not revealed, but higher biofilm inhibitory activity i.e. IC 50 at 0.025 % w / v ; and EC 50 at 0.034 % w / v were observed.

【 授权许可】

CC BY-NC