Bone Marrow Stromal Cells With Exercise and Thyroid Hormone Effect on Post-Stroke Injuries in Middle-aged Mice

Basic and Clinical Neuroscience

ARTICLE

Akhoundzadeh, Kobra¹  Vakili, Abedin¹  Sameni, Hamid Reza² 

[1] Physiology Research Center, Semnan University of Medical Sciences;Nervous System Stems Cells Research Center, Department of Anatomical Sciences, School of Medicine, Semnan University of Medical Sciences

关键词: Cerebral ischemia;    Combination;    Bone marrow stromal cells;    Thyroid hormone;    Exercise;    Apoptosis;    Glial fibrillary acidic protein;    Middle-aged;    Mice;   

DOI  :  10.32598/bcn.9.10.355

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: Based on our previous findings, the treatment of stem cells alone or in combination with thyroid hormone (T3 ) and mild exercise could effectively reduce the risk of stroke damage in young mice. However, it is unclear whether this treatment is effective in aged or middle-aged mice. Therefore, this study designed to assess whether combination of Bone Marrow Stromal Cells (BMSCs) with T3 and mild treadmill exercise can decrease stroke complications in middle-aged mice. Methods: Under laser Doppler flowmetry monitoring, transient focal cerebral ischemia was produced by right Middle Cerebral Artery Occlusion (MCAO) for 45 min followed by 7 days of reperfusion in middle-aged mice. BMSCs (1×105 ) were injected into the right cerebral ventricle 24 h after MCAO, followed by daily injection of triiodothyronine (T3 ) (20 µg/100 g/d SC) and 6 days of running on a treadmill. Infarct size, neurological function, apoptotic cells and expression levels of Glial Fibrillary Acidic Protein (GFAP) were evaluated 1 week after stroke. Results: Post-ischemic treatment with BMSCs or with T3 and or mild treadmill exercise alone or in combination did not significantly change neurological function, infarct size, and apoptotic cells 7 days after ischemia in middle-aged mice (P>0.05). However, the expression of GFAP significantly reduced after treatment with BMSCs and or T3 (P<0.01). Conclusion: Our findings indicate that post-stroke treatment BMSCs with exercise and thyroid hormone cannot reverse neuronal damage 7 days after ischemia in middle-aged mice. These findings further support that age is an important variable in stroke treatment.

【 授权许可】

Unknown