The Effect of (Aqueous-Alcoholic) Extracts and Black Pepper Essence on Vibrio cholerae and Staphylococcus aureus

International Journal of Enteric Pathogens

article

Somaye Yasliani FardYasliani Fard¹  Seyed Mahmoud Amin Marashi¹  Sahar Yasliani Fard²  Mehdi Mirzaii³  Seyed Ehsan Moosavi⁴ 

[1] Department of Microbiology and Immunology, Alborz University of Medical Sciences;Department of Bacteriology, Faculty of Medical Sciences, Damghan Branch, Islamic Azad University;Department of Microbiology, Faculty of Medical Sciences, Shahroud University of Medical Sciences, Shahroud Medical University;Department of Microbiology, Faculty of Medical Sciences, Babol University of Medical Sciences

关键词: Black Pepper;    Staphylococcus aureus;    Vibrio cholerae;   

DOI  :  10.17795/ijep21969

学科分类：农业科学（综合）

来源: Alborz University of Medical Sciences

【 摘 要 】

Background: plants been used for biological basis of drug substances in thousand years. Some of the plants have inhibitory effects on growth of intestinal infections. Black pepper is the plant that uses as classical medicine in the infections. Vibrio cholerae and Staphylococous aureus are two important bacterial agents in the food contamination. Objectives: In this study, inhibitory effects of (watery_alcoholic) extractions and essence of black pepper were studied against S. aureus and V. cholerea. Materials and Methods: Extraction produced by soaking method then for determined antibacterial effects were used cylinder method .For preparation essence 50 gram of powdered black pepper was extracted by soaking ,ethod and effect of essence was studied against the target cultuer by using the cup diffusion method. We also determined the MIC and MBC by micro dilution method. Results: Essence of black pepper showed good antibacterial effect on this bacteria. MIC of essence on the V. cholerea and S. aureus was 38 µg/ mL and 75 µg/mL, but alcohol and watery extractions didn’t have antibacterial effect on these bacteria. Conclusions: essence of black pepper showed a potent antibacterial activity and therefore, it may be used as an inhibitory extract against S. aureus and V. cholerea in food industry.

【 授权许可】

CC BY-NC