Potensi Photodinamik Inaktivasi Staphylococcus aureus dan Vibrio cholerae dengan Endogen Photosensitizer pada Penyinaran Led Biru (430 ± 4) nm dan Merah (629 ± 6) nm

Journal of Biological Researches

Suryani Dyah Astuti¹  Ni'matuzahroh¹  Djoni Izak R¹  Suhariningsih¹  M. Zainuddin² 

[1] Departemen Fisika Fsaintek Universitas Airlangga;Fakultas Farmasi Universitas Airlangga Kampus C Unair Jl. Mulyorejo Surabaya;

关键词: Photodynamic Inactivation;    porphyrin photosensitizer;    Staphylococcus aureus;    Vibrio cholerae;    Blue and red LED exposure;    Photodynamic Inactive;    Porphyrin photosensitizer;   

DOI  :  10.23869/bphjbr.16.2.20115

来源: DOAJ

【 摘 要 】

Photodynamic Inactivation (PDI) is bacteria inactivation method with using light and bacteria porphyrin photosensitizer.The combination of light and photosensitizer with suitable spectrum can promote photosensitization process and then cause bacteriaphotodamage. This research was laboratory experiment, to analyze photodamage potency of Gram positive bacteria Staphylococcusaureus and Gram negative Vibrio cholera with combination of endogen photosensitizer and LED exposure (blue LED (430 ± 4) nmand red LED (629 ± 6) nm)) on Pulse Width Modulation (PWM) 75% and time duration 30 minutes. The viability of bacteria had beencounted after 48 hours incubation on temperature 37oC by using Total Plate Count (TPC). Result of this research showed that blueLED exposure (430 ± 4) nm had potency to decrease 70% Staphylococcus aureus and 50% Vibrio cholera bacteria colony formingunit. Red LED (629 ± 6) nm exposure decreased 22% dan 3% colony forming unit. So blue LED exposure had big potency to bacteriainactivate.

【 授权许可】

Unknown