Effect of Di-ethyl aminoethyl (DEAE) Dextran on the infectivity titre of sheep pox virus in-vitro and in-vivo

Journal of Veterinary Medical Research

article

E. Nakhla, Olfat¹  A. Mohamed, Namaa¹  Awad, Manal¹  M. Ali, Mervat¹ 

[1] Veterinary Serum and Vaccine Research Institute

关键词: Effect;    Di-ethyl;    aminoethyl;    DEAE;    Dextran;    infectivity;    titre;    sheep;    pox;    virus;    Vitro;    Vivo;   

DOI  :  10.21608/jvmr.2020.77629

学科分类：社会科学、人文和艺术（综合）

来源: Beni-Suef University

【 摘 要 】

The effect of diethyl aminoethyl (DEAE) dextran on the infectivity titre of sheep pox virus (SPV) was studied with different concentrations (25, 50, 75, 100 µg/ml) of DEAE-dextran on Vero cell culture. It was found that 25 and 50µg/ml were not toxic. The same concentrations were used with sheep pox virus inoculum showing that the best virus titre (106.3 TCID50/ml) reached with the use of 25µg/ml DEAE-dextran after 10 passages. The enhanced viral fluid was tested in-vivo, by vaccination of susceptible lambs and challenge of them with the virulent sheep pox virus. These lambs showed complete protection against the disease. The SP neutralizing antibody indices (NI) were estimated in the collected serum samples post vaccination and challenge; confirmed that 25µg of DEAE-dextran/ml virus-inoculum induced an increase in neutralizing antibodies in comparison with those induced by currently used sheep pox vaccine.

【 授权许可】

CC BY