Importance of protein Ser/Thr/Tyr phosphorylation for bacterial pathogenesis

FEBS Letters

article

Julie Bonne Køhler¹  Carsten Jers¹  Mériem Senissar¹  Lei Shi²  Abderahmane Derouiche²  Ivan Mijakovic¹ 

[1] The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark;Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of Technology

关键词: antibiotic kinase inhibitors;    antibiotic resistance;    BY-kinases;    Hanks kinases;    host–pathogen interactions;    machine learning;    omics integration;    pathogenic bacteria;    phosphoproteomics;    Ser/Thr/Tyr phosphorylation;   

DOI  :  10.1002/1873-3468.13797

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein phosphorylation regulates a large variety of biological processes in all living cells. In pathogenic bacteria, the study of serine, threonine, and tyrosine (Ser/Thr/Tyr) phosphorylation has shed light on the course of infectious diseases, from adherence to host cells to pathogen virulence, replication, and persistence. Mass spectrometry (MS)-based phosphoproteomics has provided global maps of Ser/Thr/Tyr phosphosites in bacterial pathogens. Despite recent developments, a quantitative and dynamic view of phosphorylation events that occur during bacterial pathogenesis is currently lacking. Temporal, spatial, and subpopulation resolution of phosphorylation data is required to identify key regulatory nodes underlying bacterial pathogenesis. Herein, we discuss how technological improvements in sample handling, MS instrumentation, data processing, and machine learning should improve bacterial phosphoproteomic datasets and the information extracted from them. Such information is expected to significantly extend the current knowledge of Ser/ Thr/Tyr phosphorylation in pathogenic bacteria and should ultimately contribute to the design of novel strategies to combat bacterial infections.

【 授权许可】

Unknown