Umbilical cord-derived Wharton’s jelly for regenerative medicine applications in orthopedic surgery: a systematic review protocol

Journal of Orthopaedic Surgery and Research

Benjamin J. Main¹  Josiah A. Valk¹  Nicola Maffulli²  Saadiq F. El-Amin³  Manu Gupta⁴  Ian W. Stone⁵  Hugo C. Rodriguez⁶  Ashim Gupta⁷ 

[1] Beaumont Hospital Farmington Hills, Farmington Hills, MI, USA;Department of Musculoskeletal Disorders, School of Medicine and Surgery, University of Salerno, Fisciano, Italy;San Giovanni di Dio e Ruggi D’Aragona Hospital “Clinica Orthopedica” Department, Hospital of Salerno, Salerno, Italy;Queen Mary University of London, Barts and the London School of Medicine and Dentistry, Centre for Sports and Exercise Medicine, London, England;El-Amin Orthopaedic and Sports Medicine Institute, Duluth, GA, USA;BioIntegrate Inc., 2505 Newpoint Pkwy, Suite 100-A, 30043, Lawrenceville, GA, USA;Future Biologics, Lawrenceville, GA, USA;School of Osteopathic Medicine, University of the Incarnate Word, San Antonio, TX, USA;School of Osteopathic Medicine, University of the Incarnate Word, San Antonio, TX, USA;South Texas Orthopaedic Research Institute, Laredo, TX, USA;South Texas Orthopaedic Research Institute, Laredo, TX, USA;Future Biologics, Lawrenceville, GA, USA;BioIntegrate Inc., 2505 Newpoint Pkwy, Suite 100-A, 30043, Lawrenceville, GA, USA;

关键词: Regenerative medicine;    Musculoskeletal injuries;    Umbilical cord;    Wharton’s jelly;    PRISMA;   

DOI  :  10.1186/s13018-020-02067-w

来源: Springer

【 摘 要 】

BackgroundMusculoskeletal injuries and conditions affect millions of individuals. These ailments are typically managed by immobilization, physiotherapy, or activity modification. Regenerative medicine has experienced tremendous growth in the past decades, especially in musculoskeletal medicine. Umbilical cord-derived Wharton’s jelly is an exciting new option for such therapies. Wharton’s jelly is a connective tissue located within the umbilical cord largely composed of mesenchymal stem cells and extracellular matrix components, including collagen, chondroitin sulfate, hyaluronic acid, and sulfated proteoglycans. Wharton’s jelly is a promising and applicable biologic source for orthopedic regenerative application.MethodsA systematic search will be conducted in PubMed, ScienceDirect, and Google Scholar databases of English, Italian, French, Spanish, and Portuguese language articles published to date. References will be screened and assessed for eligibility by two independent reviewers as per PRISMA guidelines. Articles will be considered without exclusion to sex, activity, or age. Studies will be included if they used culture-expanded, mesenchymal stem/stromal cells of mesenchymal stem cells and/or connective tissue obtained from Wharton’s jelly. Studies will be excluded if Wharton’s jelly is not the sole experimental examined cell type. Placebos, conventional non-operative therapies including steroid injections, exercise, and NSAIDs will be compared. The study selection process will be performed independently by two reviewers using a reference software. Data synthesis and meta-analysis will be performed separately for clinical and pre-clinical studies.DiscussionThe results will be published in relevant peer-reviewed scientific journals. Investigators will present results at national or international conferences.Trial registrationThe protocol was registered on PROSPERO international prospective register of systematic reviews prior to commencement, CRD42020182487.

【 授权许可】

CC BY