Genomic and phylogenetic characterization of ChPV2, a novel goat PV closely related to the Xi-PV1 species infecting bovines

Virology Journal

Ignacio G. Bravo¹  Anouk Willemsen²  Julienne Dietz³  Alexander van den Boom³  Anja Ehrhardt³  Eric Ehrke-Schulz³  Seval Bilge Dagalp⁴  Firat Dogan⁵ 

[1] Centre National de La Recherche Scientifique (CNRS), Laboratory MIVEGEC (CNRS IRD Uni Montpellier), Montpellier, France;Center for Research on the Ecology and Evolution of Diseases (CREES), Montpellier, France;Centre National de La Recherche Scientifique (CNRS), Laboratory MIVEGEC (CNRS IRD Uni Montpellier), Montpellier, France;Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria;Chair for Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department for Human Medicine, Faculty of Health, Witten/Herdecke University, Stockumer Strasse 10, 58453, Witten, Germany;Faculty of Veterinary Medicine, Department of Virology, Ankara University, Ankara, Turkey;Faculty of Veterinary Medicine, Department of Virology, Hatay Mustafa Kemal University, Hatay, Turkey;

关键词: Novel goat papillomavirus;    ChPV2;    Genome characterization;    Phylogenetic analysis;   

DOI  :  10.1186/s12985-020-01440-9

来源: Springer

【 摘 要 】

BackgroundPapillomaviruses (PVs) infecting artiodactyls are very diverse, and only second in number to PVs infecting primates. PVs associated to lesions in economically important ruminant species have been isolated from cattle and sheep.MethodsPotential PV DNA from teat lesions of a Damascus goat was isolated, cloned and sequenced. The PV genome was analyzed using bioinformatics approaches to detect open reading frames and to predict potential features of encoded proteins as well as putative regulatory elements. Sequence comparison and phylogenetic analyses using the concatenated E1E2L2L1 nucleotide and amino acid alignments was used to reveal the relationship of the new PV to the known PV diversity and its closest relevants.ResultsWe isolated and characterized the full-genome of novel Capra hircus papillomavirus. We identified the E6, E7, E1, E2, L2, L1 open reading frames with protein coding potential and putative active elements in the ChPV2 proteins and putative regulatory genome elements. Sequence similarities of L1 and phylogenetic analyses using concatenated E1E2L2L1 nucleotide and amino acid alignments suggest the classification as a new PV type designated ChPV2 with a phylogenetic position within the XiPV genus, basal to the XiPV1 species. ChPV2 is not closely related to ChPV1, the other known goat PV isolated from healthy skin, although both of them belong confidently into a clade composed of PVs infecting cervids and bovids. Interestingly, ChPV2 contains an E6 open reading frame whereas all closely related PVs do notConclusionChPV2 is a novel goat PV closely related to the Xi-PV1 species infecting bovines. Phylogenetic relationships and genome architecture of ChPV2 and closely related PV types suggest at least two independent E6 losses within the XiPV clade.

【 授权许可】

CC BY