Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China

BMC Veterinary Research

Ming-ji Cheng¹  Yan-xia Chen¹  Yun Mo¹  Yong-zhi Li¹  Wen-qiao He¹  Ze-jin Ou¹  Yu-han Gao¹  Fang-fei You¹  Huan He¹  Min Qiu¹  Shao-wei Chen¹  Yu-qi Wen¹  Min-yi Zhang¹  Qing Chen¹  Shu-ting Huo¹  Xue-yan Zheng¹  Xue-jiao Chen¹  Xue-shan Zhong¹  Yi-quan Xiong²  Jing Ge³ 

[1] Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, 1838 Guangzhou North Road, 510515, Guangzhou, China;Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, 1838 Guangzhou North Road, 510515, Guangzhou, China;Chinese Evidence-based Medicine Center and CREAT Group, West China Hospital, Sichuan University, 610000, Chengdu, China;Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, 1838 Guangzhou North Road, 510515, Guangzhou, China;Medical Office of Wuxi People’s Hospital, 214000, Wu Xi, China;

关键词: Rattus norvegicus;    Suncus murinus;    Bacterial composition;    Next-generation sequencing;   

DOI  :  10.1186/s12917-020-02619-6

来源: Springer

【 摘 要 】

BackgroundRattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bacterial composition in these animals in China.ResultsProteobacteria, Firmicutes and Bacteroidetes were the most abundant phyla in faecal and throat swab samples of both animals. However, the composition of the bacterial community differed significantly between sample types and animal species. Firmicutes exhibited the highest relative abundance in throat swab samples of R. norvegicus, followed by Proteobacteria and Bacteroidetes. In throat swab specimens of S. murinus, Proteobacteria was the predominant phylum, followed by Firmicutes and Bacteroidetes. Firmicutes showed the highest relative abundance in faecal specimens of R. norvegicus, followed by Bacteroidetes and Proteobacteria. Firmicutes and Proteobacteria had almost equal abundance in faecal specimens of S. murinus, with Bacteroidetes accounting for only 3.07%. The family Streptococcaceae was most common in throat swab samples of R. norvegicus, while Prevotellaceae was most common in its faecal samples. Pseudomonadaceae was the predominant family in throat swab samples of S. murinus, while Enterobacteriaceae was most common in faecal samples. We annotated 33.28% sequences from faecal samples of S. murinus as potential human pathogenic bacteria, approximately 3.06-fold those in R. norvegicus. Potential pathogenic bacteria annotated in throat swab samples of S. murinus were 1.35-fold those in R. norvegicus.ConclusionsBacterial composition of throat swabs and faecal samples from R. norvegicus differed from those of S. murinus. Both species carried various pathogenic bacteria, therefore both should be closely monitored in the future, especially for S. murinus.

【 授权许可】

CC BY