Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway

Journal of Orthopaedic Surgery and Research

Yin Guan¹  Xiaoqian Zhao²  Weiwei Liu³  Yue Wang⁴ 

[1] Affiliated Hospital of Nanjing University of Chinese Medicine, 210029, Nanjing, Jiangsu, China;Department of Ethics Committee, Affiliated Hospital of Nanjing University of Chinese Medicine, 210029, Nanjing, Jiangsu, China;Department of Medical Examination Center, Affiliated Hospital of Nanjing University of Chinese Medicine, 210029, Nanjing, Jiangsu, China;Department of Rheumatism Immunity Branch, Affiliated Hospital of Nanjing University of Chinese Medicine, No. 155 Hanzhong Road, Qinhuai, 210029, Nanjing, Jiangsu, China;

关键词: Rheumatoid arthritis;    Galuteolin;    Anti-apoptosis;    Anti-inflammation;   

DOI  :  10.1186/s13018-020-02004-x

来源: Springer

【 摘 要 】

ObjectiveGaluteolin (Galu) is a substance extracted and purified from honeysuckle. The purpose of this study was to explore the effects of Galu on the TNF-α-induced RA-FLS cells (synoviocytes) and reveal its potential molecular mechanism from the perspectives of anti-apoptosis and anti-inflammation.MethodsAfter TNF-α stimulation, cell proliferation of RA-FLS was assessed by CCK-8 assay. TUNEL staining was used to detect the apoptosis. Western blot was used to detect the expressions of Iκκβ, p-p65, p65, p-IκB, IκB, Cleaved-caspase3, Caspase-3, Bcl-2, and Bax. HO-1 were determined by RT-PCR. The contents of pro-inflammatory cytokines IL-1β, IL-6, IL-8, and MMP-1 were determined by ELISA.ResultsGalu significantly suppressed cell proliferation in a dose-dependent manner. Additionally, Galu obviously promotes cell apoptosis rate of RA-FLS cells and elevated the expression levels of HO-1, caspase-3, and Bax, while reducing the expression level of Bcl-2. Furthermore, Galu apparently inhibited the levels of Iκκβ, p-p65, and p-IκB. Moreover, Galu also significantly reduced the levels of pro-inflammatory factors IL-1β, IL-6, IL-8, and MMP-1 in RA-FLS cells.ConclusionGaluteolin exerts protective effects against TNF-α-induced RA-FLS cells by inhibiting apoptosis and inflammation, which can guide the clinical use of rheumatoid arthritis.

【 授权许可】

CC BY