In vitro virulence of three Lecanicillium lecanii strains against the whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)

Egyptian Journal of Biological Pest Control

Habib Ali¹  Shah Zaman²  Tauqir Anwar³  Yusuf Ali Abdulle⁴  Azhar Uddin Keerio⁴  Trinh Duy Nam⁴  Talha Nazir⁴  Dewen Qiu⁴ 

[1] Department of Agricultural Engineering, Khawaja Fareed University of Engineering and Information Technology, 64200, Rahim Yar Khan, Punjab, Pakistan;Department of Entomology, University of Agriculture, 38000, Faisalabad, Punjab, Pakistan;Pest Warning & Quality Control of Pesticides, Punjab Agriculture Department, 40010, Sillanwali, Punjab, Pakistan;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China;

关键词: Entomopathogenic fungi;    Virulence;    Lecanicillium lecanii;    Bemisia tabaci;    Biological control;   

DOI  :  10.1186/s41938-020-00328-8

来源: Springer

【 摘 要 】

BackgroundWhitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is one of the most harmful pests in greenhouses and in open fields.Main bodyThe present study aimed to assess in vitro virulence of 3 entomopathogenic fungal strains (EPFs) of Lecanicillium lecanii 3 (V-3), 4 (V-4), and 5 (V-5) against the whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), by spray method. The 3 disparate bioassays were performed encompassed of conidial concentrations and fungal filtrate of the strains, V-3, V-4, and V-5, and also their binary combinations. In the filtrate bioassay, 2 ml of fungal filtrate of each strain was used. In the conidial bioassay, 3 disparate concentrations (1 × 105, 1 × 106, and 1 × 107 conidia ml−1) were used for each fungal strain, while in the binary combinations (1 ml filtrate + 1 ml conidia) of V-3 × V-3, V-4 × V-4, and V-5 × V-5 were used. Mortality rates against the whitefly were recorded on the 7th day. In the conidial bioassay, maximum mortality rates were found at V-3 strain (90.6%), V-4 strain (78.4%), and V-5 strain (83.6%) at the highest concentration (1 × 107 conidia ml−1) on the 7th day. In the filtrate bioassay, V-3 strain revealed a maximum mortality (93%), V-4 strain (85%), and then V-5 strain (87%) on the 7th day. Moreover, in the bioassay of binary combinations, the highest mortality rate of the whitefly was counted in V-3 × V-3 strain (84.6%), V-4 × V-4 strain (70.6%), and V-5 × V-5 strain (79.8%) on the 7th day.ConclusionAll treatments had the potential to control B. tabaci significantly. In all bioassays, the V-3 strain was the extreme virulent, and the filtrate application of V-3 strain was the utmost impressive against B. tabaci.

【 授权许可】

CC BY