Genome Medicine | |
Microbial signature in IgE-mediated food allergies | |
Yoram Louzoun1  Faiga Magzal2  Snait Tamir2  Omry Koren3  Hadar Mor3  Efrat Muller4  Elhanan Borenstein5  Dafna Magid Neriya6  Michael Y. Appel7  Liat Nachshon8  Arnon Elizur9  Ilan Youngster9  Michael R. Goldberg9  | |
[1] Department of Mathematics, Bar-Ilan University, Ramat-Gan, Israel;MIGAL-Galilee Research Institute, Kiryat Shmona, Israel;Tel-Hai College, Upper Galilee, Israel;The Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel;The Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv, Israel;The Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv, Israel;Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel;Santa Fe Institute, Santa Fe, NM, USA;The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel;Yitzhak Shamir Medical Center (Assaf Harofeh), Zerifin, Israel;Yitzhak Shamir Medical Center (Assaf Harofeh), Zerifin, Israel;Department of Medicine, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel;Yitzhak Shamir Medical Center (Assaf Harofeh), Zerifin, Israel;Department of Pediatrics, Sackler Faculty of Medicine, Tel Aviv, Israel; | |
关键词: Food allergy; Microbiota; P; SCFA; Prebiotics; Postbiotics; Supervised learning; | |
DOI : 10.1186/s13073-020-00789-4 | |
来源: Springer | |
【 摘 要 】
BackgroundMultiple studies suggest a key role for gut microbiota in IgE-mediated food allergy (FA) development, but to date, none has studied it in the persistent state.MethodsTo characterize the gut microbiota composition and short-chain fatty acid (SCFAs) profiles associated with major food allergy groups, we recruited 233 patients with FA including milk (N = 66), sesame (N = 38), peanut (N = 71), and tree nuts (N = 58), and non-allergic controls (N = 58). DNA was isolated from fecal samples, and 16S rRNA gene sequences were analyzed. SCFAs in stool were analyzed from patients with a single allergy (N = 84) and controls (N = 31).ResultsThe gut microbiota composition of allergic patients was significantly different compared to age-matched controls both in α-diversity and β-diversity. Distinct microbial signatures were noted for FA to different foods. Prevotella copri (P. copri) was the most overrepresented species in non-allergic controls. SCFAs levels were significantly higher in the non-allergic compared to the FA groups, whereas P. copri significantly correlated with all three SCFAs. We used these microbial differences to distinguish between FA patients and non-allergic healthy controls with an area under the curve of 0.90, and for the classification of FA patients according to their FA types using a supervised learning algorithm. Bacteroides and P. copri were identified as taxa potentially contributing to KEGG acetate-related pathways enriched in non-allergic compared to FA. In addition, overall pathway dissimilarities were found among different FAs.ConclusionsOur results demonstrate a link between IgE-mediated FA and the composition and metabolic activity of the gut microbiota.
【 授权许可】
CC BY
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