期刊论文详细信息
Genome Medicine
Microbial signature in IgE-mediated food allergies
Yoram Louzoun1  Faiga Magzal2  Snait Tamir2  Omry Koren3  Hadar Mor3  Efrat Muller4  Elhanan Borenstein5  Dafna Magid Neriya6  Michael Y. Appel7  Liat Nachshon8  Arnon Elizur9  Ilan Youngster9  Michael R. Goldberg9 
[1] Department of Mathematics, Bar-Ilan University, Ramat-Gan, Israel;MIGAL-Galilee Research Institute, Kiryat Shmona, Israel;Tel-Hai College, Upper Galilee, Israel;The Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel;The Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv, Israel;The Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv, Israel;Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel;Santa Fe Institute, Santa Fe, NM, USA;The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel;Yitzhak Shamir Medical Center (Assaf Harofeh), Zerifin, Israel;Yitzhak Shamir Medical Center (Assaf Harofeh), Zerifin, Israel;Department of Medicine, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel;Yitzhak Shamir Medical Center (Assaf Harofeh), Zerifin, Israel;Department of Pediatrics, Sackler Faculty of Medicine, Tel Aviv, Israel;
关键词: Food allergy;    Microbiota;    P;    SCFA;    Prebiotics;    Postbiotics;    Supervised learning;   
DOI  :  10.1186/s13073-020-00789-4
来源: Springer
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【 摘 要 】

BackgroundMultiple studies suggest a key role for gut microbiota in IgE-mediated food allergy (FA) development, but to date, none has studied it in the persistent state.MethodsTo characterize the gut microbiota composition and short-chain fatty acid (SCFAs) profiles associated with major food allergy groups, we recruited 233 patients with FA including milk (N = 66), sesame (N = 38), peanut (N = 71), and tree nuts (N = 58), and non-allergic controls (N = 58). DNA was isolated from fecal samples, and 16S rRNA gene sequences were analyzed. SCFAs in stool were analyzed from patients with a single allergy (N = 84) and controls (N = 31).ResultsThe gut microbiota composition of allergic patients was significantly different compared to age-matched controls both in α-diversity and β-diversity. Distinct microbial signatures were noted for FA to different foods. Prevotella copri (P. copri) was the most overrepresented species in non-allergic controls. SCFAs levels were significantly higher in the non-allergic compared to the FA groups, whereas P. copri significantly correlated with all three SCFAs. We used these microbial differences to distinguish between FA patients and non-allergic healthy controls with an area under the curve of 0.90, and for the classification of FA patients according to their FA types using a supervised learning algorithm. Bacteroides and P. copri were identified as taxa potentially contributing to KEGG acetate-related pathways enriched in non-allergic compared to FA. In addition, overall pathway dissimilarities were found among different FAs.ConclusionsOur results demonstrate a link between IgE-mediated FA and the composition and metabolic activity of the gut microbiota.

【 授权许可】

CC BY   

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