Analysis of correlations between gut microbiota, stool short chain fatty acids, calprotectin and cardiometabolic risk factors in postmenopausal women with obesity: a cross-sectional study

Journal of Translational Medicine

Research

Karolina Skonieczna-Żydecka¹  Igor Łoniewski²  Monika Szulińska³  Paweł Bogdański³  Daniel Styburski⁴  Konrad Podsiadło⁴  Mariusz Kaczmarczyk⁵ 

[1] Department of Biochemical Sciences, Pomeranian Medical University in Szczecin, Broniewskiego 24, 71-460, Szczecin, Poland;Department of Biochemical Sciences, Pomeranian Medical University in Szczecin, Broniewskiego 24, 71-460, Szczecin, Poland;Department of Human Nutrition and Metabolomics, Broniewskiego 24, 71-460, Szczecin, Poland;Sanprobi Sp. Z O. O. Sp. K., Kurza Stopka 5/C, 70-535, Szczecin, Poland;Department of Treatment of Obesity, Metabolic Disorders and Clinical Dietetics, University of Medical Sciences in Poznań, Szamarzewskiego Str. 84, 60-569, Poznań, Poland;Sanprobi Sp. Z O. O. Sp. K., Kurza Stopka 5/C, 70-535, Szczecin, Poland;Sanprobi Sp. Z O. O. Sp. K., Kurza Stopka 5/C, 70-535, Szczecin, Poland;Department of Clinical Biochemistry, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111, Szczecin, Poland;

关键词: Microbiota;    SCFA;    Metabolism;    Cardiometabolic risk;    Obesity;    Menopause;   

DOI  :  10.1186/s12967-022-03801-0

 received in 2022-08-12, accepted in 2022-12-01,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundMicrobiota and its metabolites are known to regulate host metabolism. In cross-sectional study conducted in postmenopausal women we aimed to assess whether the microbiota, its metabolites and gut barrier integrity marker are correlated with cardiometabolic risk factors and if microbiota is different between obese and non-obese subjects.MethodsWe analysed the faecal microbiota of 56 obese, postmenopausal women by means of 16S rRNA analysis. Stool short chain fatty acids, calprotectin and anthropometric, physiological and biochemical parameters were correlates to microbiome analyses.ResultsAlpha-diversity was inversely correlated with lipopolysaccharide (Rho = − 0.43, FDR P (Q) = 0.004). Bray–Curtis distance based RDA revealed that visceral fat and waist circumference had a significant impact on metabolic potential (P = 0.003). Plasma glucose was positively correlated with the Coriobacteriaceae (Rho = 0.48, Q = 0.004) and its higher taxonomic ranks, up to phylum (Actinobacteria, Rho = 0.46, Q = 0.004). At the metabolic level, the strongest correlation was observed for the visceral fat (Q < 0.15), especially with the DENOVOPURINE2-PWY, PWY-841 and PWY0-162 pathways. Bacterial abundance was correlated with SCFAs, thus some microbiota-glucose relationships may be mediated by propionate, as indicated by the significant average causal mediation effect (ACME): Lachnospiraceae (ACME 1.25, 95%CI (0.10, 2.97), Firmicutes (ACME 1.28, 95%CI (0.23, 3.83)) and Tenericutes (ACME − 0.39, 95%CI (− 0.87, − 0.03)). There were significant differences in the distribution of phyla between this study and Qiita database (P < 0.0001).ConclusionsMicrobiota composition and metabolic potential are associated with some CMRF and fecal SCFAs concentration in obese postmenopausal women. There is no unequivocal relationship between fecal SCFAs and the marker of intestinal barrier integrity and CMRF. Further studies with appropriately matched control groups are warranted to look for causality between SCFAs and CMRF.

【 授权许可】

CC BY   
© The Author(s) 2022

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