Combined oral intake of short and long fructans alters the gut microbiota in food allergy model mice and contributes to food allergy prevention

BMC Microbiology

Research

Kohei Funasaka¹  Tadashi Fujii²  Yoshiki Hirooka²  Takumi Tochio²  Saki Yamakawa³  Nobuhiro Kondo³  Hideaki Takahashi⁴  Kotoyo Fujiki⁴  Chikako Yamada⁴ 

[1] Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Aichi, Japan;Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Aichi, Japan;Department of Medical Research on Prebiotics and Probiotics, Fujita Health University, Toyoake, Aichi, Japan;Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Aichi, Japan;Research and Development Division, Itochu Sugar Co., Ltd., Hekinan, Aichi, Japan;WELLNEO SUGAR Co., Ltd., Tokyo, Japan;Graduate School of Nutritional Sciences, Nagoya University of Arts and Sciences, Nisshin, Aichi, Japan;

关键词: Fructan;    Food allergy;    Microbiota;    Parabacteroides;    Short-chain fatty acids;   

DOI  :  10.1186/s12866-023-03021-6

 received in 2023-06-23, accepted in 2023-09-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundIt has become clear that the intestinal microbiota plays a role in food allergies. The objective of this study was to assess the food allergy-preventive effects of combined intake of a short fructan (1-kestose [Kes]) and a long fructan (inulin ([Inu]) in an ovalbumin (OVA)-induced food allergy mouse model.ResultsOral administration of fructans lowered the allergenic symptom score and alleviated the decreases in rectal temperature and total IgA levels and increases in OVA-specific IgE and IgA levels induced by high-dose OVA challenge, and in particular, combined intake of Kes and Inu significantly suppressed the changes in all these parameters. The expression of the pro-inflammatory cytokine IL-4, which was increased in the allergy model group, was significantly suppressed by fructan administration, and the expression of the anti-inflammatory cytokine IL-10 was significantly increased upon Kes administration. 16 S rRNA amplicon sequencing of the gut microbiota and beta diversity analysis revealed that fructan administration may induce gut microbiota resistance to food allergy sensitization, rather than returning the gut microbiota to a non-sensitized state. The relative abundances of the genera Parabacteroides B 862,066 and Alloprevotella, which were significantly reduced by food allergy sensitization, were restored by fructan administration. In Parabacteroides, the relative abundances of Parabacteroides distasonis, Parabacteroides goldsteinii, and their fructan-degrading glycoside hydrolase family 32 gene copy numbers were increased upon Kes or Inu administration. The concentrations of short-chain fatty acids (acetate and propionate) and lactate were increased by fructan administration, especially significantly in the Kes + Inu, Kes, and Inu-fed (Inu, Kes + Inu) groups.ConclusionCombined intake of Kes and Inu suppressed allergy scores more effectively than single intake, suggesting that Kes and Inu have different allergy-preventive mechanisms. This indicates that the combined intake of these short and long fructans may have an allergy-preventive benefit.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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