Sensory neurons from dorsal root ganglia regulate endothelial cell function in extracellular matrix remodelling

Cell Communication and Signaling

Jacques Leng¹  Bruno Paiva dos Santos²  Joëlle Amédée²  Hugo Oliveira²  Alice Leroux² 

[1] Univ. Bordeaux, CNRS, Solvay, LOF, UMR 5258, F-33006, Pessac, France;Univ. Bordeaux, INSERM, BIOTIS, U1026, F-33000, Bordeaux, France;

关键词: Angiogenesis;    Cellular communication;    Innervation;    Matrix Metalloproteinases;    Extracellular matrix Remodelling;    Neurovascular interplay;   

DOI  :  10.1186/s12964-020-00656-0

来源: Springer

【 摘 要 】

BackgroundRecent physiological and experimental data highlight the role of the sensory nervous system in bone repair, but its precise role on angiogenesis in a bone regeneration context is still unknown. Our previous work demonstrated that sensory neurons (SNs) induce the osteoblastic differentiation of mesenchymal stem cells, but the influence of SNs on endothelial cells (ECs) was not studied.MethodsHere, in order to study in vitro the interplay between SNs and ECs, we used microfluidic devices as an indirect co-culture model. Gene expression analysis of angiogenic markers, as well as measurements of metalloproteinases protein levels and enzymatic activity, were performed.ResultsWe were able to demonstrate that two sensory neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP), were involved in the transcriptional upregulation of angiogenic markers (vascular endothelial growth factor, angiopoietin 1, type 4 collagen, matrix metalloproteinase 2) in ECs. Co-cultures of ECs with SNs also increased the protein level and enzymatic activity of matrix metalloproteinases 2 and 9 (MMP2/MMP9) in ECs.ConclusionsOur results suggest a role of sensory neurons, and more specifically of CGRP and SP, in the remodelling of endothelial cells extracellular matrix, thus supporting and enhancing the angiogenesis process.C2EPLQP2TeLXZHqSc3kN5qVideo abstract

【 授权许可】

CC BY