Targeted deletions of complement lectin pathway genes improve outcome in traumatic brain injury, with MASP-2 playing a major role

Acta Neuropathologica Communications

S. Fumagalli¹  D. Mercurio¹  M.-G. De Simoni¹  C. Perego¹  M. Oggioni¹  N. J. Lynch²  S. Ippati³  D. Minuta⁴  S. Roscher⁵  R. Wallis⁵  W. J. Schwaeble⁶ 

[1] Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, via Mario Negri 2, 20156, Milan, Italy;Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, via Mario Negri 2, 20156, Milan, Italy;Department of Veterinary Medicine, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK;Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, via Mario Negri 2, 20156, Milan, Italy;National Research Council (CNR), Institute of Neuroscience, 20129, Milan, Italy;Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, via Mario Negri 2, 20156, Milan, Italy;San Raffaele Scientific Institute, San Raffaele Hospital, 20132, Milan, Italy;Department of Respiratory Sciences, University of Leicester, University Road, LE1 9HN, Leicester, UK;Department of Veterinary Medicine, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK;

关键词: Traumatic brain injury;    Neuroinflammation;    Lectin pathway;    Complement cascade;    MBL-associated serine protease;    Neurological deficits;    Pharmacological target;   

DOI  :  10.1186/s40478-020-01041-1

来源: Springer

【 摘 要 】

The lectin pathway (LP) of complement activation is believed to contribute to brain inflammation. The study aims to identify the key components of the LP contributing to TBI outcome as possible novel pharmacological targets. We compared the long-term neurological deficits and neuropathology of wild-type mice (WT) to that of mice carrying gene deletions of key LP components after experimental TBI. WT or MASP-2 (Masp2−/−), ficolin-A (Fcna−/−), CL-11 (Colec11−/−), MASP-1/3 (Masp1−/−), MBL-C (Mbl2−/−), MBL-A (Mbl1−/−) or MBL−/− (Mbl1−/−/Mbl2−/−) deficient male C57BL/6J mice were used. Mice underwent sham surgery or TBI by controlled cortical impact. The sensorimotor response was evaluated by neuroscore and beam walk tests weekly for 4 weeks. To obtain a comparative analysis of the functional outcome each transgenic line was rated according to a health score calculated on sensorimotor performance. For selected genotypes, brains were harvested 6 weeks after injury for histopathological analysis. MASP-2−/−, MBL−/− and FCN-A−/− mice had better outcome scores compared to WT. Of these, MASP-2−/− mice had the best recovery after TBI, showing reduced sensorimotor deficits (by 33% at 3 weeks and by 36% at 4 weeks). They also showed higher neuronal density in the lesioned cortex with a 31.5% increase compared to WT. Measurement of LP functional activity in plasma from MASP-2−/− mice revealed the absence of LP functional activity using a C4b deposition assay. The LP critically contributes to the post-traumatic inflammatory pathology following TBI with the highest degree of protection achieved through the absence of the LP key enzyme MASP-2, underlining a therapeutic utility of MASP-2 targeting in TBI.

【 授权许可】

CC BY