Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST

BMC Veterinary Research

Nor Fitriah Mohamed Sohaimi¹  Latiffah Hassan²  Rohaya Mohd Ali³  Zawiyah Sharif⁴  Shafini Abu Bakar⁴  Nor Hazrin binti Abd Hazis⁵  Suraya Amir Husin⁵  Norazah Ahmad⁵  Zunita Zakaria⁶  Bashiru Garba⁷ 

[1] Bacteriology Laboratory, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Bacteriology Laboratory, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Veterinary Laboratory Diagnostics, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Diagnostic and Quality Assurance Division, Department of Veterinary Services, Ministry of Agriculture & Agro-Based Industry, Putrajaya, Malaysia;Food Safety and Quality Division, Ministry of Health, 62675, Putrajaya, Malaysia;Infectious Diseases Research Centre, Institute for Medical Research, National Institutes of Health, Setia Alam, Selangor, Malaysia;Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Bacteriology Laboratory, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sultan Abubakar Road, City Campus Complex, 840212, Sokoto, Sokoto State, Nigeria;

关键词: Salmonella;    Chicken meat products;    Pulse field gel electrophoresis;    Multi-locus sequence typing;    Whole-genome sequencing;    Antimicrobial resistance;   

DOI  :  10.1186/s12917-020-02605-y

来源: Springer

【 摘 要 】

BackgroundSalmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates. Upon serotyping of the isolates, the antimicrobial susceptibility profiling using a panel of 9 commonly used antimicrobials was done. Subsequently, the molecular profiles of all the isolates were further determined using Pulsed Field Gel Electrophoresis (PFGE) and the Whole Genome Multi-Locus Sequence Type (wgMLST) analysis in order to obtain the sequence types.ResultsThe PFGE data was input into FPQuest software, and the dendrogram generated was studied for possible genetic relatedness among the isolates. All the isolates were found to belong to the Salmonella Enteritidis serotype with notable resistance to tetracycline, gentamycin, streptomycin, and sulfadimidine. The S. Enteritidis isolates tested predominantly subtyped into the ST11 and ST1925, which was found to be a single cell variant of ST11. The STs were found to occur in chicken meats, foods, and live chicken cloacal swabs, which may indicate the persistence of the bacteria in multiple foci.ConclusionThe data demonstrate the presence of S. Enteritidis among chickens, indicating its preference and reservoir status for enteric Salmonella pathogens.

【 授权许可】

CC BY