Phylogenetic grouping, epidemiological typing, analysis of virulence genes, and antimicrobial susceptibility of Escherichia coli isolated from healthy broilers in Japan

Irish Veterinary Journal

Tetsuo Asai³  Akemi Kojima²  Hideto Sekiguchi⁴  Saiki Imamura⁴  Mai Tsuyuki⁴  Michiko Kawanishi²  Tetsuo Akiyama²  Masaru Usui¹  Mototaka Hiki² 

[1] Laboratory of Food Microbiology and Food Safety, Department of Health and Environmental Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan;National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan;The United Graduate School of Veterinary Sciences, Gifu University, Gifu-shi, Gifu, Japan;Food Safety and Consumer Affairs Bureau, Ministry of Agriculture, Forestry and Fisheries, 1-2-1 Kasumigaseki, Chiyoda-ku, Tokyo 100-8950, Japan

关键词: Antimicrobial resistance;    Virulence-associated gene;    Phylogenetic grouping;    Multi-locus sequence typing;    Escherichia coli;    Broiler;   

Others  :  1135902
DOI  :  10.1186/2046-0481-67-14

 received in 2014-02-27, accepted in 2014-06-20,  发布年份 2014

【 摘 要 】

Background

The aim of our study was to investigate the possible etiology of avian colibacillosis by examining Escherichia coli isolates from fecal samples of healthy broilers.

Findings

Seventy-eight E. coli isolates from fecal samples of healthy broilers in Japan were subjected to analysis of phylogenetic background, virulence-associated gene profiling, multi-locus sequence typing (MLST), and antimicrobial resistance profiling. Phylogenetic analysis demonstrated that 35 of the 78 isolates belonged to group A, 28 to group B1, one to group B2, and 14 to group D. Virulence-associated genes iutA, iss, cvaC, tsh, iroN, ompT, and hlyF were found in 23 isolates (29.5%), 16 isolates (20.5%), nine isolates (11.5%), five isolates (6.4%), 19 isolates (24.4%), 23 isolates (29.5%), and 22 isolates (28.2%) respectively. Although the genetic diversity of group D isolates was revealed by MLST, the group D isolates harbored iutA (10 isolates, 71.4%), iss (6 isolates, 42.9%), cvaC (5 isolates, 35.7%), tsh (3 isolates, 21.4%), hlyF (9 isolates, 64.3%), iroN (7 isolates, 50.0%), and ompT (9 isolates, 64.3%).

Conclusions

Our results indicated that E. coli isolates inhabiting the intestines of healthy broilers pose a potential risk of causing avian colibacillosis.

【 授权许可】

   
2014 Hiki et al.; licensee BioMed Central Ltd.

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