LINC01006 facilitates cell proliferation, migration and invasion in prostate cancer through targeting miR-34a-5p to up-regulate DAAM1

Cancer Cell International

Qianqian Wang¹  Xinqi Zhang²  Enhui Ma³  Zhenjia Guo³  Xiaofeng Yang⁴  Jinhua Li⁵ 

[1] Department of Nephrology, Zaozhuang Municipal Hospital, 277100, Zaozhuang, Shandong, China;Department of Urology, Shandong Zibo Mining Group Central Hospital, 255120, Zibo, Shandong, China;Department of Urology, Southwest Shandong Hospital Co., Ltd, 252300, Liaocheng, Shandong, China;Department of Urology, Zaozhuang Municipal Hospital, NO.41 Longtou Road, Shizhong District, 277100, Zaozhuang, Shandong, China;Orthopeadic Surgery, Southwest Shandong Hospital Co., Ltd, 252300, Liaocheng, Shandong, China;

关键词: LINC01006;    miR-34a-5p;    DAAM1;    Prostate cancer;   

DOI  :  10.1186/s12935-020-01577-1

来源: Springer

【 摘 要 】

BackgroundProstate cancer (PCa) is a kind of malignancy occurring in the prostate gland. Substantial researches have proved the major role of long noncoding RNAs (lncRNAs) in PCa. However, the role of long intergenic non-protein coding RNA 1006 (LINC01006) in PCa has not been investigated yet.MethodsRT-qPCR was used to examine the expression levels of LINC01006 and its downstream targets. The function of LINC01006 in PCa was tested by in vitro and in vivo assays. With application of RNA pull down, RNA immunoprecipitation (RIP) and luciferase reporter assays, the interaction among LINC01006, miR-34a-5p and disheveled associated activator of morphogenesis 1 (DAAM1) were verified.ResultsLINC01006 expression presented high in PCa cell lines. LINC01006 silencing suppressed cell proliferative, migratory, invasive capacities while accelerated apoptotic rate. Besides, LINC01006 knockdown also suppressed tumor growth and metastasis in vivo. Furthermore, miR-34a-5p, a tumor suppressor in PCa, was sponged by LINC01006. Moreover, DAAM1 was targeted by miR-34a-5p and promoted PCa progression. More intriguingly, rescue assays suggested that the inhibitory effect of LINC01006 knockdown on PCa development was offset by DAAM1 overexpression.ConclusionsLINC01006 promoted PCa progression by sponging miR-34a-5p to up-regulate DAAM1, providing a novel target for PCa therapy.

【 授权许可】

CC BY