期刊论文详细信息
Frontiers in Cell and Developmental Biology
The simultaneous administration of microplastics and cadmium alters rat testicular activity and changes the expression of PTMA, DAAM1 and PREP
Cell and Developmental Biology
Sergio Minucci1  Massimo Venditti1  Majida Ben Hadj Hassine2  Imed Messaoudi2 
[1] Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate “F. Bottazzi”, Università degli Studi della Campania “Luigi Vanvitelli”, Naples, Italy;Laboratoire LR11ES41 Génétique Biodiversité et Valorisation des Bio-ressourcés, Institut Supérieur de Biotechnologie de Monastir, Université de Monastir, Monastir, Tunisia;
关键词: microplastic;    cadmium;    endocrine disrupters;    prothymosin alpha;    cytoskeleton;    DAAM1;    PREP;    testis;   
DOI  :  10.3389/fcell.2023.1145702
 received in 2023-01-16, accepted in 2023-02-27,  发布年份 2023
来源: Frontiers
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【 摘 要 】

This paper confirms the damaging effects produced by MP and Cd on testicular activity in the rat. Oral treatment with both chemicals resulted in testicular damage, documented by biomolecular and histological alterations, particularly by impaired morphometric parameters, increased apoptosis, reduced testosterone synthesis, and downregulation of the steroidogenic enzyme 3β-HSD. We also demonstrated, for the first time, that both MP and Cd can affect the protein level of PTMA, a small peptide that regulates germ cell proliferation and differentiation. Interestingly, the cytoarchitecture of testicular cells was also altered by the treatments, as evidenced by the impaired expression and localization of DAAM1 and PREP, two proteins involved in actin- and microtubule-associated processes, respectively, during germ cells differentiation into spermatozoa, impairing normal spermatogenesis. Finally, we showed that the effect of simultaneous treatment with MP and Cd were more severe than those produced by MP alone and less harmful than those of Cd alone. This could be due to the different ways of exposure of the two substances to rats (in drinking water for Cd and in oral gavage for MP), since being the first contact in the animals’ gastrointestinal tract, MP can adsorb Cd, reducing its bioavailability through the Trojan-horse effect.

【 授权许可】

Unknown   
Copyright © 2023 Venditti, Ben Hadj Hassine, Messaoudi and Minucci.

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