Immunoprecipitation and mass spectrometry define TET1 interactome during oligodendrocyte differentiation

Cell & Bioscience

Kaixiang Zhang¹  Xianghui Zhao¹  Ming Zhang¹  Jian Wang¹  Yuming Liu¹  Shengxi Wu¹  Guangxin Liu¹  Weilin Jin² 

[1] Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, 710032, Xi’an, Shaanxi, China;School of Electronic, Information and Electrical Engineering, Shanghai Jiao Tong University, 200240, Shanghai, China;

关键词: Oligodendrocyte;    TET1;    DNA dioxygenase;    Mass spectrometry;    Co-immunoprecipitation;   

DOI  :  10.1186/s13578-020-00473-5

来源: Springer

【 摘 要 】

Ten-eleven translocation (TET) proteins, encoding dioxygenase for DNA hydroxymethylation, are important players in nervous system development and disease. In addition to their proverbial enzymatic role, TET proteins also possess non-enzymatic activity and function in multiple protein–protein interaction networks, which remains largely unknown during oligodendrocyte differentiation. To identify partners of TET1 in the myelinating cells, we performed proteome-wide analysis using co-immunoprecipitation coupled to mass spectrometry (IP-MS) in purified oligodendrocyte precursor cells (OPCs) and mature oligodendrocytes (mOLs), respectively. Following a stringent selection of MS data based on identification reliability and protein enrichment, we identified a core set of 1211 partners that specifically interact with TET1 within OPCs and OLs. Analysis of the biological process and pathways associated with TET1-interacting proteins indicates a significant enrichment of proteins involved in regulation of cellular protein localization, cofactor metabolic process and regulation of catabolic process, et al. We further validated TET1 interactions with selected partners. Overall, this comprehensive analysis of the endogenous TET1 interactome during oligodendrocyte differentiation suggest its novel mechanism in regulating oligodendrocyte homeostasis and provide comprehensive insight into the molecular pathways associated with TET1.

【 授权许可】

CC BY