Comparative analysis of sucrose phosphate synthase (SPS) gene family between Saccharum officinarum and Saccharum spontaneum

BMC Plant Biology

Qing Zhang¹  Lanping Chen¹  Xingtan Zhang¹  Panpan Ma¹  Xiuting Hua¹  Haibao Tang¹  Jisen Zhang²  Qingyi Yu³  Zhengchao Wang⁴  Ray Ming⁵  Muqing Zhang⁶  Qian Zhao⁷ 

[1] Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Crop Science, Fujian Agriculture and Forestry University, 350002, Fuzhou, China;Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Crop Science, Fujian Agriculture and Forestry University, 350002, Fuzhou, China;Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning, Guangxi, China;Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Crop Science, Fujian Agriculture and Forestry University, 350002, Fuzhou, China;Texas A&M AgriLife Research, Department of Plant Pathology and Microbiology, Texas A&M University System, 75252, Dallas, TX, USA;College of Life Sciences, Fujian Normal University, 350007, Fuzhou, China;Department of Plant Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA;Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning, Guangxi, China;Institute of Applied Ecology, Fujian Agriculture and Forestry University, 350002, Fuzhou, China;

关键词: Sugarcane;    S. officinarum;    S. spontaneum;    Sucrose phosphate synthase (SPS);    Polyploidy;    BAC libraries;    Transcriptome;    Metabolites;   

DOI  :  10.1186/s12870-020-02599-7

来源: Springer

【 摘 要 】

BackgroundSucrose phosphate synthase (SPS) genes play vital roles in sucrose production across various plant species. Modern sugarcane cultivar is derived from the hybridization between the high sugar content species Saccharum officinarum and the high stress tolerance species Saccharum spontaneum, generating one of the most complex genomes among all crops. The genomics of sugarcane SPS remains under-studied despite its profound impact on sugar yield.ResultsIn the present study, 8 and 6 gene sequences for SPS were identified from the BAC libraries of S. officinarum and S. spontaneum, respectively. Phylogenetic analysis showed that SPSD was newly evolved in the lineage of Poaceae species with recently duplicated genes emerging from the SPSA clade. Molecular evolution analysis based on Ka/Ks ratios suggested that polyploidy reduced the selection pressure of SPS genes in Saccharum species. To explore the potential gene functions, the SPS expression patterns were analyzed based on RNA-seq and proteome dataset, and the sugar content was detected using metabolomics analysis. All the SPS members presented the trend of increasing expression in the sink-source transition along the developmental gradient of leaves, suggesting that the SPSs are involved in the photosynthesis in both Saccharum species as their function in dicots. Moreover, SPSs showed the higher expression in S. spontaneum and presented expressional preference between stem (SPSA) and leaf (SPSB) tissue, speculating they might be involved in the differentia of carbohydrate metabolism in these two Saccharum species, which required further verification from experiments.ConclusionsSPSA and SPSB genes presented relatively high expression and differential expression patterns between the two Saccharum species, indicating these two SPSs are important in the formation of regulatory networks and sucrose traits in the two Saccharum species. SPSB was suggested to be a major contributor to the sugar accumulation because it presented the highest expressional level and its expression positively correlated with sugar content. The recently duplicated SPSD2 presented divergent expression levels between the two Saccharum species and the relative protein content levels were highest in stem, supporting the neofunctionalization of the SPSD subfamily in Saccharum.

【 授权许可】

CC BY