Salivary microbiome in patients undergoing hemodialysis and its associations with the duration of the dialysis

BMC Nephrology

Xiaolei Chen¹  Xi Tang¹  Dutmanee Seriwatanachai²  Anchun Mo³  Megha Gupta⁴  Dan Li⁵  Xuedong Zhou⁵  Qiuchan Xiong⁵  Hanxiao Xue⁵  Tong Xu⁵  Yuqing Li⁵  Xiaobo Duan⁵  Quan Yuan⁶ 

[1] Department of Nephrology, West China Hospital, Sichuan University, Chengdu, China;Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand;Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China;Department of Preventive Dental Sciences, Division of Pedodontics, College of Dentistry, Al-Showajra Academic Campus, Jazan University, Jazan, Kingdom of Saudi Arabia;State Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Renmin South Road, 610041, Chengdu, Sichuan, China;State Key Laboratory of Oral Diseases & National Clinical Research Centre for Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Renmin South Road, 610041, Chengdu, Sichuan, China;Department of Preventive Dental Sciences, Division of Pedodontics, College of Dentistry, Al-Showajra Academic Campus, Jazan University, Jazan, Kingdom of Saudi Arabia;

关键词: Saliva;    Oral microbiome;    Hemodialysis;    Periodontal disease;   

DOI  :  10.1186/s12882-020-02009-y

来源: Springer

【 摘 要 】

BackgroundChronic kidney disease (CKD) patients, especially those with end stage renal disease (ESRD) undergoing hemodialysis (HD), exhibit high prevalence of periodontitis. This cross-sectional study aimed to investigate the periodontal status of HD patients and its relationship with salivary microbiome.MethodsOne hundred eight HD patients and one hundred healthy control individuals were recruited. They were subjected to periodontal examination followed by saliva samples collection for 16S rRNA gene sequencing.ResultsThe HD patients were with worse periodontal health status, and exhibited higher salivary microbial diversity and lower richness. The periodontal pathogens were significantly enriched in the HD patients. The inferred functional analyze showed microbes enriched in the HD patients were mainly related to metabolism. Despite the periodontal status and overall structure of the microbiome were not significantly altered as the HD duration prolonged, the abundance of Lachnospiraceae [G-2] sp. |HMT_096| is positively correlated with the duration of HD and the community periodontal index (CPI). Five OTUs (operational taxonomic units) belonging to the phyla Firmicutes were enriched as the duration prolonged, and four OTUs originated from the phyla Proteobacteria were negatively related with the CPI index. ESRD patients undergoing HD exhibited microbiota structural, compositional and functional differences compared with the healthy controls. And the species changed as the duration of hemodialysis prolonged.ConclusionsEnd stage renal disease changes salivary microbiome and is a risk factor for oral dysbiosis.

【 授权许可】

CC BY