Identifying Aggregatibacter actinomycetemcomitans periodontal antigens by immunoscreening.

Oral biology;Periodontitis;A. actinomycetemcomitans;Periodontal disease

Gerald Bernard Pevow

University:University of Louisville

Department:Oral Biology

关键词: Oral biology;    Periodontitis;    A. actinomycetemcomitans;    Periodontal disease;   

Others  :  https://ir.library.louisville.edu/cgi/viewcontent.cgi?article=2123&context=etd

美国|英语

来源: The Universite of Louisville's Institutional Repository

【 摘 要 】

Periodontitis is a chronic, destructive inflammatory disease of the supportingtissues of the teeth with a high prevalence among adults. While the completepathogenesis of periodontitis remains unclear, it is initiated and sustained by dentalplaque containing pathogens such as Porphyromonas gingivalis, Aggregatibacteractinomycetemcomitans, Bacteroides forsythus, and Prevotella intermedia.Our hypothesis is that multiple antigens are recognized by human antibodies, andantigens can be identified by use of a genomic expression library. We developed anunbiased global approach to define A. actinomycetemcomitans protein antigens that elicithumoral immune responses and developed a screening method to identify periodontalantigens. We identified 19 unique antigens, 17 of which have not been studied in A.actinomycetemcomitans. Future studies should 1) use additional human sera to identify additional antigens, 2) elucidate the role of identified antigens, and 3) determine how A.actinomycetemcomitans antigen-antibody profiles relate to disease progression.