Neutrophils mediated multistage nanoparticle delivery for prompting tumor photothermal therapy

Journal of Nanobiotechnology

Kun Wang¹  Bao Zhao²  Jianou Qiao³  Yilong Guo⁴  Qinguo Lu⁴  Bo Ye⁵  Ang Li⁶  Longpo Zheng⁷ 

[1] Cancer Center, Shanghai East Hospital, Tongji University, Shanghai, China;Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu, China;Department of Respiratory Medicine, Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Rd, 200011, Shanghai, China;Department of Thoracic Surgery, Pizhou people’s hospital, Xuzhou Medical University, Pizhou, China;Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China;School of Life Science and Technology, Tongji University, 1239 Siping Road, 200092, Shanghai, People’s Republic of China;Shanghai Tenth People’s Hospital, School of Medicine, Tongji University, 200072, Shanghai, People’s Republic of China;

关键词: Neutrophils;    Photothermal therapy;    Inflammatory signal;    Au nanorod;    Tumour homing;   

DOI  :  10.1186/s12951-020-00682-7

来源: Springer

【 摘 要 】

BackgroundNeutrophil-based drug delivery system possesses excellent advantages in targeting at tumour because neutrophils are easily recruited by chemotactic factor in tumor microenvironment. Herein, we developed a novel tactic of multistage neutrophils-based nanoparticle delivery system for promoting photothermal therapy (PTT) of lung cancer.ResultsAu nanorod (AuNR) was successfully modified with bovine serum albumin (AuNRB) and further conjugated with RGD (AuNRBR), followed by neutrophil internalisation to obtain neutrophils-based delivery system (AuNRBR/N). The engineered neutrophils efficiently migrated across the epithelial cells due to inflammatory signal. They exhibited better toxicity against Lewis cells with laser irradiation in vitro. Moreover, AuNRBR/N showed significantly more targetability to tumour tissue compared with cell carrier-free AuNRBR, as demonstrated in Lewis tumour-bearing mice. The enhanced tumour homing efficiency of AuNRBR/N together with subsequently released AuNRBR from the neutrophils was favourable for further deep tissue diffusion and contributed to the inhibition of the tumour growth in PTT and improved survival rate (over 120 days).ConclusionsOverall results illustrated that the design of cell-based nanoparticle delivery system for PTT of cancer is promising.

【 授权许可】

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