Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

Brazilian Journal of Medical and Biological Research

F.s. Martins¹  J.j.h. Celestino¹  M.v.a. Saraiva¹  R.n. Chaves¹  R. Rossetto¹  C.m.g. Silva¹  I.b. Lima-verde¹  C.a.p. Lopes¹  C.c. Campello¹  J.r. Figueiredo¹ 

[1] ,Universidade Estadual do Ceará Faculdade de Veterinária Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antraisFortaleza CE ,Brasil

关键词: GDF-9;    IGF-I;    GH;    Culture;    Follicle activation;    Goat;   

DOI  :  10.1590/S0100-879X2010007500066

来源: SciELO

【 摘 要 】

The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a significant and concomitant increase in the percent of intermediate and primary follicles in all treatments tested after 7 days of culture when compared to non-cultured tissue. After 7 days of culture, the highest percent of intermediate follicles was observed with IGF-I/GH (61.3%), and the highest percent of primary follicles was achieved with IGF-I (57.7%). After 7 days of culture in MEM+ containing GDF-9, IGF-I and GH alone or in all associations, a significant increase in follicular diameter was observed when compared to MEM+ alone and non-cultured tissue. In conclusion, GDF-9, IGF-I and GH alone or in combination maintain preantral follicle survival and promote primordial follicle activation. Nevertheless, the data showed that IGF-I/GH and IGF-I alone are efficient in promoting the transition from primordial to intermediate follicles and from intermediate to primary follicles, respectively.

【 授权许可】

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