Effects of insulin and IGF-I on growth hormone- induced STAT5 activation in 3T3-F442A adipocytes

Lipids in Health and Disease

Xiangdong Wang⁴  Stuart J Frank¹  Jing Jiang⁵  Qingbo Guan²  Wenjie Zhang³  Weina Gao⁶  Xia Li⁶  Yuantao Liu³  Yuchao Zhang⁶ 

[1] Endocrinology Section, Medical Service, Veterans Affairs Medical Center, Birmingham, AL, 35233, UK;Shandong Provincial Hospital, affiliated to Shandong University, Jinan, 250021, China;The Second Hospital of Shandong University, Jinan, 250033, China;Key Laboratory of Protein Sciences for Chronic Degenerative Diseases in Universities of Shandong (Shandong University), Jinan 250033, China;Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Alabama at Birmingham, Birmingham, AL, 35294, UK;The Institute of Cell Biology, Shandong University School of Medicine, Jinan 250012, China

关键词: IGF-I;    3T3-F442A adipocyte;    Insulin;    GH;   

Others  :  834758
DOI  :  10.1186/1476-511X-12-56

 received in 2013-02-27, accepted in 2013-04-18,  发布年份 2013

【 摘 要 】

Background

Growth hormone (GH) and insulin signaling pathways are known important regulators of adipose homeostasis. The cross-talk between GH and insulin signaling pathways in mature adipocytes is poorly understood.

Methods

In the present study, the impact of insulin on GH-mediated signaling in differentiated 3T3-F442A adipocytes and primary mice adipocytes was examined.

Results

Insulin alone did not induce STAT5 tyrosine phosphorylation, but enhanced GH-induced STAT5 activation. This effect was more pronounced when insulin was added 20 min prior to GH treatment. The above results were further confirmed by in vivo study, showing that insulin pretreatment potentiated GH- induced STAT5 tyrosine phosphorylation in visceral adipose tissues of C57/BL6 mice. In addition, our in vitro results showed that IGF-I had similar potentiating effect as insulin on GH-induced STAT5 activation. In vitro, insulin and IGF-I had an additive effect on GH- induced MAPK activation.

Conclusion

These results indicate that both insulin and IGF-I specifically potentiated GH mediated STAT5 activation in mature adipose cells. These findings suggest that insulin and GH, usually with antagonistic functions, might act synergistically to regulate some specific functions in mature adipocytes.

【 授权许可】

   
2013 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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