Effects of insulin and IGF-I on growth hormone- induced STAT5 activation in 3T3-F442A adipocytes

Lipids in Health and Disease

Research

Jing Jiang¹  Stuart J Frank²  Qingbo Guan³  Xia Li⁴  Yuchao Zhang⁴  Weina Gao⁴  Xiangdong Wang⁵  Yuantao Liu⁶  Wenjie Zhang⁶ 

[1] Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Alabama at Birmingham, 35294, Birmingham, AL, UK;Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Alabama at Birmingham, 35294, Birmingham, AL, UK;Endocrinology Section, Medical Service, Veterans Affairs Medical Center, 35233, Birmingham, AL, UK;Shandong Provincial Hospital, affiliated to Shandong University, 250021, Jinan, China;The Institute of Cell Biology, Shandong University School of Medicine, 250012, Jinan, China;The Institute of Cell Biology, Shandong University School of Medicine, 250012, Jinan, China;Key Laboratory of Protein Sciences for Chronic Degenerative Diseases in Universities of Shandong (Shandong University), 250033, Jinan, China;The Second Hospital of Shandong University, 250033, Jinan, China;

关键词: GH;    Insulin;    3T3-F442A adipocyte;    IGF-I;   

DOI  :  10.1186/1476-511X-12-56

 received in 2013-02-27, accepted in 2013-04-18,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundGrowth hormone (GH) and insulin signaling pathways are known important regulators of adipose homeostasis. The cross-talk between GH and insulin signaling pathways in mature adipocytes is poorly understood.MethodsIn the present study, the impact of insulin on GH-mediated signaling in differentiated 3T3-F442A adipocytes and primary mice adipocytes was examined.ResultsInsulin alone did not induce STAT5 tyrosine phosphorylation, but enhanced GH-induced STAT5 activation. This effect was more pronounced when insulin was added 20 min prior to GH treatment. The above results were further confirmed by in vivo study, showing that insulin pretreatment potentiated GH- induced STAT5 tyrosine phosphorylation in visceral adipose tissues of C57/BL6 mice. In addition, our in vitro results showed that IGF-I had similar potentiating effect as insulin on GH-induced STAT5 activation. In vitro, insulin and IGF-I had an additive effect on GH- induced MAPK activation.ConclusionThese results indicate that both insulin and IGF-I specifically potentiated GH mediated STAT5 activation in mature adipose cells. These findings suggest that insulin and GH, usually with antagonistic functions, might act synergistically to regulate some specific functions in mature adipocytes.

【 授权许可】

Unknown   
© Zhang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.