Ocorrência de genes tad associados à formação de biofilme em isolados de Pasteurella multocida de pulmões de suínos com pneumonia

Pesquisa Veterinária Brasileira

Danny Franciele Da S.d. Moraes²  Laila Natasha S. Brandão²  Leticia C. Pitchenin²  João Xavier O. Filho¹  Nelson Morés¹  Luciano Nakazato²  Valéria Dutra² 

[1] ,Universidade Federal do Mato Grosso Hospital Veterinário Departamento de Clínica Médica VeterináriaCuiabá MT ,Brasil

关键词: Swine;    Pasteurella multocida;    gene tad;    pneumonia;    Suínos;    Pasteurella multocida;    genes tad;    pneumonia;   

DOI  :  10.1590/S0100-736X2014001200001

来源: SciELO

【 摘 要 】

Nowadays pig intensive production systems increase microbial selection pressure favoring spread of respiratory diseases. The bacteria Pasteurella multocida is associated with various respiratory diseases in pigs under farming conditions causing high economic losses. In vitro biofilm formation has been described in P. multocida which role is described in tissue colonization, enhancing resistance to host defenses and antibiotics. The aims of this study were to analyze the occurrence of P. multocida in pig pneumonia and health lung microbiota, and occurrence of tad locus genes in these isolates. Seventy isolates of P. multocida were analyzed which sixty seven were from lungs with lesion and three from healthy lungs. Serotype A occurred mainly in lung lesion (85.71%), in healthy lung, instead, only serotype D was observed. Genes tadA, tadB, tadC, tadD, tadE, tadF and tadG were present in 89.55% isolates from lesion lung. Genes tadA, tadB and tadC were present in all isolates from healthy lungs but, tadD, tadE tadF and tadG were present in 0%, 33.3%, 33,3% and 66.6%, respectively. This work associated tadD, tadE and tadF gene positive isolates of P. multocida to lung pneumonic lesions.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License