Detection and phylogenetic analysis of porcine enteric calicivirus, genetically related to the Cowden strain of sapovirus genogroup III, in Brazilian swine herds

Pesquisa Veterinária Brasileira

Aline F. Barry¹  Alice F. Alfieri¹  Amauri A. Alfieri¹ 

[1] ,Universidade Estadual de Londrina Centro de Ciências Agrárias Departamento de Medicina Veterinária PreventivaLondrina PR ,Brazil

关键词: Swine;    piglets;    diarrhea;    calicivirus;    Sapovirus;    Suínos;    leitões;    diarréia;    calicivírus;    Sapovirus;   

DOI  :  10.1590/S0100-736X2008000100013

来源: SciELO

【 摘 要 】

Sapovirus of the Caliciviridae family is an important agent of acute gastroenteritis in children and piglets. The Sapovirus genus is divided into seven genogroups (G), and strains from the GIII, GVI and GVII are associated with infections in swine. Despite the high prevalence in some countries, there are no studies related to the presence of porcine enteric sapovirus infections in piglets in Brazil. In the present study, 18 fecal specimens from piglets up to 28 days were examined to determine the presence of sapovirus genome by RT-PCR assay, using primers designed to amplify a 331 bp segment of the RNA polymerase gene. In 44.4% (8/18) of fecal samples, an amplified DNA fragment was obtained. One of these fragments was sequenced and submitted to molecular and phylogenetic analysis. This analysis revealed high similarity, with nucleotides (87%) and amino acids (97.8%), to the Cowden strain, the GIII prototype of porcine enteric calicivirus. This is the first description of sapovirus in Brazilian swine herds.

【 授权许可】

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