Pesquisa Veterinária Brasileira | |
Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite | |
Sandra M. Borowski2  Nilo Ikuta1  Vagner Lunge1  André Fonseca1  Edmundo Marques1  Marisa Cardoso1  | |
[1] ,Fepagro Centro de Pesquisas Veterinárias Desidério Finamor Porto Alegre RS | |
关键词: Pasteurella multocida; pigs; pneumonia; pleuritis; Pasteurella multocida; suínos; pneumonia; pleurite; | |
DOI : 10.1590/S0100-736X2002000300002 | |
来源: SciELO | |
【 摘 要 】
The antigenic and phenotypic variation among 22 strains of Pasteurella multocida isolated from pig lungs with pneumonia and/or pleuritic lesions was studied. Phenotypic tests consisted of biochemical assays and antimicrobial sensitivity tests. All isolates fermented mannitol and sorbitol, but none fermented arabinose. Fourteen fermented xylose, 4 trehalose, 2 dulcitol and 1 maltose. Analyzing these characteristics, 5 different biochemical groups were revealed. There was a wide variation in the results of antimicrobial sensitivity testing to 9 products, with 50% of isolates showing sensitivity at least to 7. No antimicrobial agent was able to inhibit all strains. The highest efficiency (72% sensitivity) was observed with amoxycillin (30 mg). Spectinomycin (100 mg) presented the lowest efficiency, 45.5%. Antigenic characterization consisted in capsular serotyping and assessment of variability of an outer membrane protein gene (ompH), using polymerase chain reaction (PCR) and digestion with 5 endonuclease enzymes (restriction fragment length polymorphism, RFLP). Out of 22 strains, 21 were classified as capsular type A and one as type D. Characterization of ompH revealed 7 different patterns. They corresponded well to previously established biochemical groups.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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