Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite

Pesquisa Veterinária Brasileira

Sandra M. Borowski²  Nilo Ikuta¹  Vagner Lunge¹  André Fonseca¹  Edmundo Marques¹  Marisa Cardoso¹ 

[1] ,Fepagro Centro de Pesquisas Veterinárias Desidério Finamor Porto Alegre RS

关键词: Pasteurella multocida;    pigs;    pneumonia;    pleuritis;    Pasteurella multocida;    suínos;    pneumonia;    pleurite;   

DOI  :  10.1590/S0100-736X2002000300002

来源: SciELO

【 摘 要 】

The antigenic and phenotypic variation among 22 strains of Pasteurella multocida isolated from pig lungs with pneumonia and/or pleuritic lesions was studied. Phenotypic tests consisted of biochemical assays and antimicrobial sensitivity tests. All isolates fermented mannitol and sorbitol, but none fermented arabinose. Fourteen fermented xylose, 4 trehalose, 2 dulcitol and 1 maltose. Analyzing these characteristics, 5 different biochemical groups were revealed. There was a wide variation in the results of antimicrobial sensitivity testing to 9 products, with 50% of isolates showing sensitivity at least to 7. No antimicrobial agent was able to inhibit all strains. The highest efficiency (72% sensitivity) was observed with amoxycillin (30 mg). Spectinomycin (100 mg) presented the lowest efficiency, 45.5%. Antigenic characterization consisted in capsular serotyping and assessment of variability of an outer membrane protein gene (ompH), using polymerase chain reaction (PCR) and digestion with 5 endonuclease enzymes (restriction fragment length polymorphism, RFLP). Out of 22 strains, 21 were classified as capsular type A and one as type D. Characterization of ompH revealed 7 different patterns. They corresponded well to previously established biochemical groups.

【 授权许可】

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