ELISA de bloqueio monoclonal para o diagnóstico sorológico de infecções pelo herpesvírus bovino tipo 1 (BHV-1)

Pesquisa Veterinária Brasileira

Marjorie F.b. Teixeira¹  Paulo A. Esteves¹  Cyntia S. Schmidt¹  Fernando R. Spilki¹  Tamir C. Silva¹  Maria A. Dotta¹  Paulo M. Roehe¹ 

[1] ,Fundação Estadual de Pesquisa Agropecuária Centro de Pesquisa Veterinária Desidério Finamor Eldorado do Sul RS

关键词: Bovine herpesvirus;    BHV-1;    infectious bovine rhinotracheitis virus;    ELISA;    monoclonal;    Herpesvírus bovino tipo 1;    rinotraqueíte infecciosa bovina;    BHV-1;    ELISA monoclonal;   

DOI  :  10.1590/S0100-736X2001000100007

来源: SciELO

【 摘 要 】

A monoclonal antibody-based blocking enzyme linked immunosorbent assay (ELISA-M) was developed and standardized for the detection of antibodies to infectious bovine rhinotracheitis virus (Bovine Herpesvirus type 1; BHV-1). A total of 266 samples of bovine sera (148 negative and 118 positive) were tested and compared with the results of a standard serum neutralization (SN) test. The ELISA-M was adjusted to 92.37% sensitivity, 92.56% especificity, 93.83% negative predictive value, 90.83% positive predictive value and to an accuracy of 92.48%, with an agreement index (k) equal to 0.85. The main advantages presented by the ELISA-M were its practicality and rapidity in performance. This test was shown to be a suitable alternative to SN tests in the detection of BHV-1 antibodies in cattle. However, the ELISA was unable to discriminate between BHV-1 and bovine herpesvirus type 5 (BHV-5) antibodies.

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