Monoclonal antibody differentiation between bovine herpesviruses type 1 and 5

Pesquisa Veterinária Brasileira

Roehe, Paulo Michel¹  Oliveira, Liliane Guimarães¹  Nardi, Nance Beyer³  Silva, Tamir Calcagnotto da²  Rosa, Júlio César de Almeida¹ 

[1] Fundação Estadual de Pesquisa Agropequária, , Porto Alegre,;IRFA Biotecnologia Industrial Ltda, Porto Alegre;Universidade Federal doRio Grande do Sul

关键词: Bovine herpesviruses;    infectious bovine rhinotracheitis virus;    IBR;    IPV;    bovine encephalitis virus;    BHV-1;    BHV-5.;   

DOI  :  10.1590/S0100-736X1997000100007

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

.- Roehe P.M., Silva T.C., Nardi N.B., Oliveira L.G. & Rosa J.C.A. 1997. [Monoclonal antibody differentiation between bovine herpesviruses type 1 and 5.] Diferenciação entre os vírus da rinotraqueíte infecciosa bovina (BHV-1) e herpesvírus da encefalite bovina (BHV-5) com anticorpos monoclonais. Pesquisa Veterinária Brasileira 17(1):41-44. Centro de Pesquisas Veterinárias Desidério Finamor, Caixa Postal 2076, Porto Alegre, RS 90001-970, Brazil.Bovine Herpesviruses (BHV) type 1 (BHV-1) and type 5 (BHV-5) were analysed by immunoperoxidase staining with a panel of monoclonal antibodies (Mabs) prepared against BHV antigens. One of the Mabs recognized all BHV isolates tested. The remainder four mabs recognized only BHV-1 samples, including standard laboratory strains. All isolates associated with clinical cases of encephalitis (BHV-5) displayed a pattern of reactivity distinct from that of viruses isolated from syndromes associated with BHV-1 infections. The results obtained indicate that such Mabs allowed the differentiation between BHV-1 and BHV-5, with a perfect correlation between the clinical pictures and the patterns of reactivity in vitro. INDEX

【 授权许可】

CC BY-NC