DIFERENCIAÇÃO ENTRE OS VÍRUS DA RINOTRAQUEÍTE INFECCIOSA BOVINA (BHV-1) E HERPESVÍRUS DA ENCEFALITE BOVINA (BHV-5) COM ANTICORPOS MONOCLONAIS

Pesquisa Veterinária Brasileira

Paulo Michel Roehe²  Tamir Calcagnotto Da Silva¹  Nance Beyer Nardi¹  Liliane Guimarães Oliveira²  Júlio César De Almeida Rosa² 

[1] ,Fundação Estadual de Pesquisa Agropequária, Porto Alegre, RS

关键词: Bovine herpesviruses;    infectious bovine rhinotracheitis virus;    IBR;    IPV;    bovine encephalitis virus;    BHV-1;    BHV-5;    Herpesvírus bovinos;    rinotraqueíte infecciosa bovina;    encefalite bovina;    BHV-1;    BHV-5;   

DOI  :  10.1590/S0100-736X1997000100007

来源: SciELO

【 摘 要 】

Bovine Herpesviruses (BHV) type 1 (BHV-1) and type 5 (BHV-5) were analysed by immunoperoxidase staining with a panel of monoclonal antibodies (Mabs) prepared against BHV antigens. One of the Mabs recognized all BHV isolates tested. The remainder four mabs recognized only BHV-1 samples, including standard laboratory strains. All isolates associated with clinical cases of encephalitis (BHV-5) displayed a pattern of reactivity distinct from that of viruses isolated from syndromes associated with BHV-1 infections. The results obtained indicate that such Mabs allowed the differentiation between BHV-1 and BHV-5, with a perfect correlation between the clinical pictures and the patterns of reactivity in vitro.

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