A monoclonal blocking ELISA for the serological diagnosis of bovine herpesvirus type 1 (BHV-1) infections

Pesquisa Veterinária Brasileira

Teixeira, Marjorie F.B.¹  Silva, Tamir C.¹  Schmidt, Cyntia S.¹  Spilki, Fernando R.¹  Dotta, Maria A.¹  Esteves, Paulo A.¹  Roehe, Paulo M.¹ 

[1] Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul

关键词: Bovine herpesvirus;    BHV-1;    infectious bovine rhinotracheitis virus;    ELISA;    monoclonal.;   

DOI  :  10.1590/S0100-736X2001000100007

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

.- Teixeira M.F.B., Esteves, P.A, Schmidt, C.S., Dotta M.A. & Roehe, P.M. 2001. [A monoclonal blocking ELISA for the serological diagnosis of bovine herpesvirus type 1 (BHV-1) infections] ELISA de bloqueio monoclonal para o diagnóstico sorológico de infecções pelo herpesvírus bovino tipo 1 (BHV-1). Pesquisa Veterinária Brasileira 21(1):33-37. Centro de Pesquisa Veterinária Desidério Finamor (CPVDF), FEPAGRO, Cx. Postal 2076, Porto Alegre, RS 90001-970, Brazil. A monoclonal antibody-based blocking enzyme linked immunosorbent assay (ELISA-M) was developed and standardized for the detection of antibodies to infectious bovine rhinotracheitis virus (Bovine Herpesvirus type 1; BHV-1). A total of 266 samples of bovine sera (148 negative and 118 positive) were tested and compared with the results of a standard serum neutralization (SN) test. The ELISA-M was adjusted to 92.37% sensitivity, 92.56% especificity, 93.83% negative predictive value, 90.83% positive predictive value and to an accuracy of 92.48%, with an agreement index (k) equal to 0.85. The main advantages presented by the ELISA-M were its practicality and rapidity in performance. This test was shown to be a suitable alternative to SN tests in the detection of BHV-1 antibodies in cattle. However, the ELISA was unable to discriminate between BHV-1 and bovine herpesvirus type 5 (BHV-5) antibodies.INDEX

【 授权许可】

CC BY-NC