CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

Revista Árvore

Laureen Michelle Houllou¹  Robson Antônio De Souza¹  Elizabete Cristina Pacheco Dos Santos¹  José Jackson Pereira Da Silva¹  Marta Ribeiro Barbosa¹  Jean Philippe Guimarães Sauvé¹  Wolfgang Harand¹ 

关键词: Micropropagation;    In vitro developmental characteristics;    Callus organogenesis;    Micropropagação;    Características do desenvolvimento in vitro;    Organogêneses via calo;   

DOI  :  10.1590/0100-67622015000300004

来源: SciELO

【 摘 要 】

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.

【 授权许可】

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