Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants

Química Nova

Luciana Cristina Vitorino²  Fabiano Guimarães Silva²  William Cardoso Lima¹  Marcos Antônio Soares¹  Rita Cássia Nascimento Pedroso¹  Marolí Rodrigues Silva¹  Herbert Júnior Dias¹  Antônio Eduardo Miller Crotti¹  Márcio Luís Andrade E Silva¹  Wilson Roberto Cunha¹  Patrícia Mendonça Pauletti¹  Ana Helena Januário¹ 

[1] ,Instituto Federal de Educação, Ciência e Tecnologia GoianoRio Verde GO ,Brasil

关键词: in vitro culture;    Hyptis marrubioides;    Lamiaceae;   

DOI  :  10.1590/S0100-40422013000700016

来源: SciELO

【 摘 要 】

Hyptis marrubioides Epling is a native plant from Brazilian Cerrado. In this paper, the response of in vitro microplants of this species to inoculation with bacterial and fungal endophytic isolates is evaluated. HPLC-DAD analysis showed the presence of 3,4-O-(Z)-dicaffeoylquinic acid and quercetin-7-O-glucoside as the main components. GC/MS analysis demonstrated that the sesquiterpenes τ-cadinol and caryophyllene oxide were only produced in microplants inoculated with endophytic bacteria, while methyl hexadecanoate, methyl heptadecanoate and methyl (Z,Z,Z) 9,12,15-octadecatrienoate and the triterpene methyl 3β-hydroxy-urs-12-en-28-oate were overexpressed only when the microplant was treated with endophytic fungi.

【 授权许可】

CC BY-NC   
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